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KCC2 antibody

Anti-KCC2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133130
Anti-KCC2 antibody used in Western Blot (WB). GTX133130
Anti-KCC2 antibody used in Western Blot (WB). GTX133130
Anti-KCC2 antibody used in Western Blot (WB). GTX133130
Anti-KCC2 antibody used in Western Blot (WB). GTX133130
Anti-KCC2 antibody used in Western Blot (WB). GTX133130
Anti-KCC2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133130
Anti-KCC2 antibody used in Western Blot (WB). GTX133130
Anti-KCC2 antibody used in Western Blot (WB). GTX133130
Anti-KCC2 antibody used in Western Blot (WB). GTX133130

Cat. No. GTX133130

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all KCC2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

126 kDa. ( Note )

Positive Control

SK-N-SH , mouse hippocampus , rat hippocampus , rat spinal cord

Predict Reactivity

Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.92 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of mouse KCC2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886837

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

solute carrier family 12, member 5 , KCC2 , mKIAA1176

Cellular Localization

Cell projection , dendrite , Cell membrane; Multi-pass membrane protein

Background

Mediates electroneutral potassium-chloride cotransport in mature neurons. Transport occurs under isotonic conditions, but is activated 20-fold by cell swelling. Important for Cl(-) homeostasis in neurons.

Database

Research Area

DATA IMAGES

Anti-KCC2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133130

GTX133130 IHC-P Image

KCC2 antibody detects KCC2 protein expression by immunohistochemical analysis.
Sample:Paraffin-Embedded adult mouse retina.
Green: KCC2 protein stained by KCC2 antibody (GTX133130) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-KCC2 antibody used in Western Blot (WB). GTX133130

GTX133130 WB Image

Human brain (5 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with KCC2 antibody (GTX133130) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-KCC2 antibody used in Western Blot (WB). GTX133130

GTX133130 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with KCC2 antibody (GTX133130) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-KCC2 antibody used in Western Blot (WB). GTX133130

GTX133130 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with KCC2 antibody (GTX133130) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-KCC2 antibody used in Western Blot (WB). GTX133130

GTX133130 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with KCC2 antibody (GTX133130) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 29028184

Anti-KCC2 antibody used in Western Blot (WB). GTX133130

GTX133130 WB Image

Rat tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with KCC2 antibody (GTX133130) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 29028184

Anti-KCC2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133130

GTX133130 ICC/IF Image

KCC2 antibody detects KCC2 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary hippocampal neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: KCC2 protein stained by KCC2 antibody (GTX133130) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-KCC2 antibody used in Western Blot (WB). GTX133130

GTX133130 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with KCC2 antibody (GTX133130) diluted at 1:500.

Anti-KCC2 antibody used in Western Blot (WB). GTX133130

GTX133130 WB Image

Rat tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with KCC2 antibody (GTX133130) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 29028184

Anti-KCC2 antibody used in Western Blot (WB). GTX133130

GTX133130 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with KCC2 antibody (GTX133130) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Wang S et al. Neurosci Lett 2022; 775 : 136536 Combination of repetitive transcranial magnetic stimulation and treadmill training reduces hyperreflexia by rebalancing motoneuron excitability in rats after spinal cord contusion.
Application : IHC-P
Reactivity : Rat
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169