Boiled and unboiled various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with KCNIP4 antibody [GT178] (GTX643016) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Corresponding RNA expression data are based on NCBI database.

Boiled and unboiled rat tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with KCNIP4 antibody [GT178] (GTX643016) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.