GeneTex
United States (US)

KCNN2 antibody, Internal

GTX88269_2422_WB_18122102_695.jpg
GTX88269_1011_IHC-P_18122102_958.jpg

Cat No. GTX88269

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.3-1μg/ml
IHC-P 1-2μg/ml

Note :

IHC-P
Humam Thyroid Gland shows membranous staining in the epithelial cells.

Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88269-PEP).

Specificity/Sensitivity

This antibody is expected to recognize both isoforms (NP_067627.2; NP_740721.1).

Predict Reactivity

Mouse, Rat, Bovine, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-ESYDKHVTYNAER, from the internal region of the protein sequence according to NP_067627.2; NP_740721.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Potassium Calcium-Activated Channel Subfamily N Member 2,Kca2.2,Sk2,Skca2,Skca 2,Hsk2,Kcnn2

Cellular Localization

Membrane; Multi-pass membrane protein

Background

Action potentials in vertebrate neurons are followed by an afterhyperpolarization (AHP) that may persist for several seconds and may have profound consequences for the firing pattern of the neuron. Each component of the AHP is kinetically distinct and is mediated by different calcium-activated potassium channels. The protein encoded by this gene is activated before membrane hyperpolarization and is thought to regulate neuronal excitability by contributing to the slow component of synaptic AHP. This gene is a member of the KCNN family of potassium channel genes. The encoded protein is an integral membrane protein that forms a voltage-independent calcium-activated channel with three other calmodulin-binding subunits. Alternate splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013]

Database

DATA IMAGES
GTX88269_2422_WB_18122102_695.jpg

GTX88269 WB Image

WB analysis of human liver lysate using GTX88269 KCNN2 antibody, Internal.
Dilution : 0.3µg/ml
Loading : 35µg protein in RIPA buffer

GTX88269_1011_IHC-P_18122102_958.jpg

GTX88269 IHC-P Image

IHC-P analysis of human thyroid Gland using GTX88269 KCNN2 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8µg/ml

Package List Price ($)
$ 329