*Optimal dilutions/concentrations should be determined by the researcher.
Humam Thyroid Gland shows membranous staining in the epithelial cells.
Not tested in other applications.
The peptide used to generate this antibody is available for purchase (GTX88269-PEP).
This antibody is expected to recognize both isoforms (NP_067627.2; NP_740721.1).
Mouse, Rat, Bovine, Dog(>80% identity)
Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Peptide with sequence C-ESYDKHVTYNAER, from the internal region of the protein sequence according to NP_067627.2; NP_740721.1.
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Potassium Calcium-Activated Channel Subfamily N Member 2,Kca2.2,Sk2,Skca2,Skca 2,Hsk2,Kcnn2
Membrane; Multi-pass membrane protein
Action potentials in vertebrate neurons are followed by an afterhyperpolarization (AHP) that may persist for several seconds and may have profound consequences for the firing pattern of the neuron. Each component of the AHP is kinetically distinct and is mediated by different calcium-activated potassium channels. The protein encoded by this gene is activated before membrane hyperpolarization and is thought to regulate neuronal excitability by contributing to the slow component of synaptic AHP. This gene is a member of the KCNN family of potassium channel genes. The encoded protein is an integral membrane protein that forms a voltage-independent calcium-activated channel with three other calmodulin-binding subunits. Alternate splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013]
GTX88269 WB Image
WB analysis of human liver lysate using GTX88269 KCNN2 antibody, Internal.
Dilution : 0.3µg/ml
Loading : 35µg protein in RIPA buffer
GTX88269 IHC-P Image
IHC-P analysis of human thyroid Gland using GTX88269 KCNN2 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8µg/ml