APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.3-1μg/ml |
| 1-2μg/ml |
|
Note :
IHC-P
Humam Thyroid Gland shows membranous staining in the epithelial cells.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX88269-PEP).
Product Note
This antibody is expected to recognize both isoforms (NP_067627.2; NP_740721.1).
Predict Reactivity
Mouse, Rat, Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-ESYDKHVTYNAER, from the internal region of the protein sequence according to NP_067627.2; NP_740721.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
potassium calcium-activated channel subfamily N member 2 , KCa2.2 , SK2 , SKCA2 , SKCa 2 , hSK2
Cellular Localization
Membrane; Multi-pass membrane protein
Background
Action potentials in vertebrate neurons are followed by an afterhyperpolarization (AHP) that may persist for several seconds and may have profound consequences for the firing pattern of the neuron. Each component of the AHP is kinetically distinct and is mediated by different calcium-activated potassium channels. The protein encoded by this gene is activated before membrane hyperpolarization and is thought to regulate neuronal excitability by contributing to the slow component of synaptic AHP. This gene is a member of the KCNN family of potassium channel genes. The encoded protein is an integral membrane protein that forms a voltage-independent calcium-activated channel with three other calmodulin-binding subunits. Alternate splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013]
Database
Research Area
DATA IMAGES
|
GTX88269 WB Image
WB analysis of human liver lysate using GTX88269 KCNN2 antibody, Internal.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer
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GTX88269 IHC-P Image
IHC-P analysis of human thyroid Gland using GTX88269 KCNN2 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8μg/ml
|
REFERENCE
There are currently no references for KCNN2 antibody, Internal (GTX88269). Be the first to share your publications with this product.
REVIEW
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