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KCNN3 antibody

Anti-KCNN3 antibody used in Western Blot (WB). GTX54779
Anti-KCNN3 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54779

Cat No. GTX54779

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-Fr

Reactivity

Human, Mouse, Rat
Package
50 μl ($549)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

81 kDa. ( Note )

Predict Reactivity

Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 1% BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.6 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide DTSGHFHDSGVGDLDEDPKC, corresponding to amino acid residues 2-21 (Intracellular, N-terminus) of human KCNN3 (Accession  : Q9UGI6).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887887

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

potassium calcium-activated channel subfamily N member 3 , KCa2.3 , SK3 , SKCA3 , hSK3

Cellular Localization

Membrane; Multi-pass membrane protein

Background

Action potentials in vertebrate neurons are followed by an afterhyperpolarization (AHP) that may persist for several seconds and may have profound consequences for the firing pattern of the neuron. Each component of the AHP is kinetically distinct and is mediated by different calcium-activated potassium channels. This gene belongs to the KCNN family of potassium channels. It encodes an integral membrane protein that forms a voltage-independent calcium-activated channel, which is thought to regulate neuronal excitability by contributing to the slow component of synaptic AHP. This gene contains two CAG repeat regions in the coding sequence. It was thought that expansion of one or both of these repeats could lead to an increased susceptibility to schizophrenia or bipolar disorder, but studies indicate that this is probably not the case. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]

Database

Research Area

DATA IMAGES

Anti-KCNN3 antibody used in Western Blot (WB). GTX54779

GTX54779 WB Image

WB analysis of rat brain membrane lysate using GTX54779 KCNN3 antibody preincubated with or without immunogen peptide.
Dilution : 1:200

Anti-KCNN3 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54779

GTX54779 IHC-Fr Image

IHC-Fr analysis of mouse dopaminergic neurons tissue using GTX54779 KCNN3 antibody. Triangles point at cells with co-localization.
Panel A : KCa2.3 is detected  in  substantia nigra pars compacta.
Panel B : Tyrosine hydroxylase staining shows dopaminergic neurons.

REFERENCE

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf