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KCTD15 antibody [AT4C3]

Anti-KCTD15 antibody [AT4C3] used in Western Blot (WB). GTX50002
Anti-KCTD15 antibody [AT4C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50002

Cat No. GTX50002

Host

Mouse

Clonality

Monoclonal

Clone Name

AT4C3

Isotype

IgG3

Application

WB, ICC/IF, ELISA

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

In Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human KCTD15 (1-234) purified from E.coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Potassium Channel Tetramerization Domain Containing 15 , Kctd15

Background

KCTD15 (Potassium channel tetramerisation domain containing 15, also known as BTB/POZ domain-containing protein KCTD15) is protein that in humans is encoded by the KCTD15 gene. KCTD15 is expressed at high level in brain and hypothalamus. The potassium channel KCTD15 was identified as a genetic loci associated with higher than normal body mass index (BMI) in humans along with genes such as GNPDA2, MTCH2, FTO, and TMEM18. Single nucleotide polymorphisms (SNPs) in non-diabetic and diabetic patients showed that FTO was most strongly associated with obesity while MTCH2 and GNPDA2 were still significantly associated with higher than normal BMI levels.

Database

Research Area

DATA IMAGES

Anti-KCTD15 antibody [AT4C3] used in Western Blot (WB). GTX50002

GTX50002 WB Image

Cell lysates of HeLa (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human KCTD15 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Anti-KCTD15 antibody [AT4C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50002

GTX50002 ICC/IF Image

Immunofluorescence of human HeLa cells stained with monoclonal anti-human KCDT15 antibody (1:500) with Texas Red (Red). Nucleus was stained by Hoechst 33342 (Blue).

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 319