GeneTex

  • Country / Location Selection

United States (US)

KDEL antibody

Anti-KDEL antibody used in Flow cytometry (FACS). GTX22898
Anti-KDEL antibody used in IHC (Paraffin sections) (IHC-P). GTX22898
Anti-KDEL antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22898
Anti-KDEL antibody used in IHC (Paraffin sections) (IHC-P). GTX22898
Anti-KDEL antibody used in IHC (Paraffin sections) (IHC-P). GTX22898

Cat. No. GTX22898

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS, IP, IHC

Reactivity

 Human, Mouse, Rat, Hamster
Package
100 μg ($399)
See all KDEL products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 8-16 μg/ml
ICC/IF Assay dependent
IHC-P 1:100-200
FACS 1-20 μg/ml
IP Assay dependent
IHC Assay dependent
Not tested in other applications.

Product Note

This antibody detects a 57 kDa protein representing protein disulfide isomerase (PDI), a 78 kDa protein representing glucose regulated protein 70 kDa (GRP78) and an ~94 kDa protein representing GRP94 in mouse myeloma cells.

PROPERTIES

Form

Liquid

Buffer

buffer

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Synthetic peptide corresponding to residues C T(643) G E E D T S E K D E L(654) of rat GRP94.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-KDEL antibody used in Flow cytometry (FACS). GTX22898

GTX22898 FACS Image

FACS analysis of permeabilized, fixed HeLa cells using GTX22898 KDEL antibody.
Purple histogram : Primary antibody
Black histogram : Isotype control antibody
Dilution : 10 μg/ml incubated for 1 hour on ice

Anti-KDEL antibody used in IHC (Paraffin sections) (IHC-P). GTX22898

GTX22898 IHC-P Image

IHC-P analysis of mouse pancreas tissue using GTX22898 KDEL antibody.
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100

Anti-KDEL antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22898

GTX22898 ICC/IF Image

ICC/IF analysis of U251 cells using GTX22898 KDEL antibody. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

Anti-KDEL antibody used in IHC (Paraffin sections) (IHC-P). GTX22898

GTX22898 IHC-P Image

IHC-P analysis of mouse lymph node tissue using GTX22898 KDEL antibody.
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

Anti-KDEL antibody used in IHC (Paraffin sections) (IHC-P). GTX22898

GTX22898 IHC-P Image

IHC-P analysis of mouse liver tissue using GTX22898 KDEL antibody.
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100

REFERENCE

There are currently no references for KDEL antibody (GTX22898). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for KDEL antibody (GTX22898). Be the first to share your experience with this product.
Submit a Review
SDS
Sodium Azide.pdf
Package List Price ($)
$ 399