APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Human, Mouse, Rat, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.73 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human KDEL receptor. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1241003
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
KDEL endoplasmic reticulum protein retention receptor 1 , ERD2 , ERD2.1 , HDEL , PM23
Cellular Localization
Endoplasmic reticulum membrane; Multi-pass membrane protein
Background
Retention of resident soluble proteins in the lumen of the endoplasmic reticulum (ER) is achieved in both yeast and animal cells by their continual retrieval from the cis-Golgi, or a pre-Golgi compartment. Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually lys-asp-glu-leu (KDEL) in animal cells, and his-asp-glu-leu (HDEL) in S. cerevisiae. This process is mediated by a receptor that recognizes, and binds the tetrapeptide-containing protein, and returns it to the ER. In yeast, the sorting receptor encoded by a single gene, ERD2, which is a seven-transmembrane protein. Unlike yeast, several human homologs of the ERD2 gene, constituting the KDEL receptor gene family, have been described. The protein encoded by this gene was the first member of the family to be identified, and it encodes a protein structurally and functionally similar to the yeast ERD2 gene product. [provided by RefSeq]
Database
Research Area
REFERENCE
There are currently no references for KDEL receptor antibody [N1C1] (GTX106417). Be the first to share your publications with this product.
REVIEW
KDEL receptor antibody [N1C1]
Cat. No. GTX106417
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Rating
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( Average 5 based on 1 users reviews)
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Application
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Western Blot(WB)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 12-Dec-2013
Application Tested :
WB
Sample Species :
Hm
Sample :
BHK-21 cells
Amount used :
30μg
Blocking :
GeneTex Universal Protein Blocking Reagent (GTX30963), 25°C, 0.17Hr
Primary Antibody :
1:1000, 4°C, 16Hr
![Anti-KDEL receptor antibody [N1C1] used in Western Blot (WB). GTX106417](/upload/website/support/rewardsprogram/normal/genetex_GTX106417_WB_19080419_c9ffdc65-9415-4b13-ae4b-1ce5172353e39595.jpg "Anti-KDEL receptor antibody [N1C1] used in Western Blot (WB). GTX106417")