GeneTex
United States (US)

KDEL receptor antibody [N1C1]

Cat No. GTX106417

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Hamster
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

*Hamster BHK-21 cells ,

Predict Reactivity

Human, Mouse, Rat, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.729mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human KDEL receptor. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ERD2 antibody, ERD2.1 antibody, HDEL antibody, PM23 antibody, KDELR1 antibody, ER lumen protein retaining receptor 1 antibody, KDEL endoplasmic reticulum protein retention receptor 1 antibody, putative MAPK-activating protein PM23 antibody, KDEL receptor 1 antibody, KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 1 antibody

Cellular Localization

Endoplasmic reticulum membrane; Multi-pass membrane protein

Background

Retention of resident soluble proteins in the lumen of the endoplasmic reticulum (ER) is achieved in both yeast and animal cells by their continual retrieval from the cis-Golgi, or a pre-Golgi compartment. Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually lys-asp-glu-leu (KDEL) in animal cells, and his-asp-glu-leu (HDEL) in S. cerevisiae. This process is mediated by a receptor that recognizes, and binds the tetrapeptide-containing protein, and returns it to the ER. In yeast, the sorting receptor encoded by a single gene, ERD2, which is a seven-transmembrane protein. Unlike yeast, several human homologs of the ERD2 gene, constituting the KDEL receptor gene family, have been described. The protein encoded by this gene was the first member of the family to be identified, and it encodes a protein structurally and functionally similar to the yeast ERD2 gene product. [provided by RefSeq]

Database

Research Area

REVIEW

KDEL receptor antibody [N1C1] Cat No. GTX106417

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 12-Dec-2013
Score :
Application Tested : WB
Sample Species : Hm
Sample : BHK-21 cells
Amount used : 30μg
Blocking : GeneTex Universal Protein Blocking Reagent (GTX30963), 25°C, 0.17Hr
Primary Antibody : 1:1000, 4°C, overnightHr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169