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KDM3A antibody

Anti-KDM3A antibody used in Western Blot (WB). GTX129046
Anti-KDM3A antibody used in Immunoprecipitation (IP). GTX129046

Cat No. GTX129046

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

147 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human KDM3A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

lysine demethylase 3A , JHDM2A , JHMD2A , JMJD1 , JMJD1A , TSGA

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a zinc finger protein that contains a jumonji domain and may play a role in hormone-dependent transcriptional activation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]

Database

Research Area

DATA IMAGES

Anti-KDM3A antibody used in Western Blot (WB). GTX129046

GTX129046 WB Image

KDM3A antibody detects KDM3A protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5 % SDS-PAGE, and blotted with KDM3A antibody (GTX129046) diluted by 1:2000

Anti-KDM3A antibody used in Immunoprecipitation (IP). GTX129046

GTX129046 IP Image

KDM3A antibody immunoprecipitates KDM3A protein in IP experiments.
IP samples: 293T whole cell extract
A. 50 μg 293T whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of KDM3A protein by 4 μg KDM3A antibody (GTX129046)
5 % SDS-PAGE
The immunoprecipitated KDM3A protein was detected by KDM3A antibody (GTX129046) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

REFERENCE

There are currently no references for KDM3A antibody (GTX129046). Be the first to share your publications with this product.

REVIEW

KDM3A antibody Cat No. GTX129046

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 26-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Amount used : 30 μg
Blocking : % non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:100, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169