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KDM3A antibody

Anti-KDM3A antibody used in Western Blot (WB). GTX129046
Anti-KDM3A antibody used in Immunoprecipitation (IP). GTX129046

Cat No. GTX129046

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

147 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human KDM3A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885881

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

lysine demethylase 3A , JHDM2A , JHMD2A , JMJD1 , JMJD1A , TSGA

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a zinc finger protein that contains a jumonji domain and may play a role in hormone-dependent transcriptional activation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]

Database

Research Area

DATA IMAGES

Anti-KDM3A antibody used in Western Blot (WB). GTX129046

GTX129046 WB Image

KDM3A antibody detects KDM3A protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5 % SDS-PAGE, and blotted with KDM3A antibody (GTX129046) diluted by 1:2000

Anti-KDM3A antibody used in Immunoprecipitation (IP). GTX129046

GTX129046 IP Image

KDM3A antibody immunoprecipitates KDM3A protein in IP experiments.
IP samples: 293T whole cell extract
A. 50 μg 293T whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of KDM3A protein by 4 μg KDM3A antibody (GTX129046)
5 % SDS-PAGE
The immunoprecipitated KDM3A protein was detected by KDM3A antibody (GTX129046) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

REFERENCE

There are currently no references for KDM3A antibody (GTX129046). Be the first to share your publications with this product.

REVIEW

KDM3A antibody Cat No. GTX129046

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 26-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Amount used : 30 μg
Blocking : % non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:100, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169