APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1 μg/mL |
| 5 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
HepG2 Cell Lysate(GTX27900)
Product Note
This antibody will not cross-react with JMJD1B or JMJD1C.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
KDM3A antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human KDM3A.The immunogen is located within amino acids 50 - 100 of KDM3A.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
lysine demethylase 3A , JHDM2A , JHMD2A , JMJD1 , JMJD1A , TSGA
Cellular Localization
Cytoplasm,Nucleus
Background
The jumonji domain containing 1A protein (JMJD1A) was originally discovered as a testes specific gene, but has been found to be expressed in numerous tissues. JMJD1A is a histone demethylase and specifically demethylates mono- and dimethyl-H3K9. It has also been found to be a novel interaction partner with ER71, a transcription factor expressed in the testes of adult mice and during embryogenesis. JMJD1A is also a downstream gene of STAT3, a protein that has been found to be important in the maintenance of mouse embryonic stem (mES) cells, and decreased JMJD1A expression correlated with the differentiation of cultured mES cells following the removal of the cytokine LIF. These findings suggest that JMJD1A might be a critical signaling molecule underlying the maintenance of pluripotency in embryonic stem cells. At least two isoforms of JMJD1A are known to exist. This antibody will not cross-react with JMJD1B or JMJD1C.
Database
Research Area
DATA IMAGES
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GTX85253 IHC-P Image
IHC-P analysis of rat liver tissue using GTX85253 KDM3A antibody.
Working concentration : 20 μg/ml
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GTX85253 IHC-P Image
IHC-P analysis of rat liver tissue using GTX85253 KDM3A antibody.
Working concentration : 5 μg/ml
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GTX85253 WB Image
WB analysis of HepG2 cell lysate using GTX85253 KDM3A antibody.
Working concentration : 1 μg/ml
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REFERENCE
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REVIEW
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