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KDM3A antibody

Anti-KDM3A antibody used in Western Blot (WB). GTX85253
Anti-KDM3A antibody used in IHC (Paraffin sections) (IHC-P). GTX85253
Anti-KDM3A antibody used in IHC (Paraffin sections) (IHC-P). GTX85253

Cat. No. GTX85253

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all KDM3A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

147 kDa. ( Note )

Positive Control

HepG2 Cell Lysate(GTX27900)

Product Note

This antibody will not cross-react with JMJD1B or JMJD1C.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KDM3A antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human KDM3A.The immunogen is located within amino acids 50 - 100 of KDM3A.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lysine demethylase 3A , JHDM2A , JHMD2A , JMJD1 , JMJD1A , TSGA

Cellular Localization

Cytoplasm,Nucleus

Background

The jumonji domain containing 1A protein (JMJD1A) was originally discovered as a testes specific gene, but has been found to be expressed in numerous tissues. JMJD1A is a histone demethylase and specifically demethylates mono- and dimethyl-H3K9. It has also been found to be a novel interaction partner with ER71, a transcription factor expressed in the testes of adult mice and during embryogenesis. JMJD1A is also a downstream gene of STAT3, a protein that has been found to be important in the maintenance of mouse embryonic stem (mES) cells, and decreased JMJD1A expression correlated with the differentiation of cultured mES cells following the removal of the cytokine LIF. These findings suggest that JMJD1A might be a critical signaling molecule underlying the maintenance of pluripotency in embryonic stem cells. At least two isoforms of JMJD1A are known to exist. This antibody will not cross-react with JMJD1B or JMJD1C.

Database

Research Area

DATA IMAGES

Anti-KDM3A antibody used in Western Blot (WB). GTX85253

GTX85253 WB Image

WB analysis of HepG2 cell lysate using GTX85253 KDM3A antibody.
Working concentration : 1 μg/ml

Anti-KDM3A antibody used in IHC (Paraffin sections) (IHC-P). GTX85253

GTX85253 IHC-P Image

IHC-P analysis of rat liver tissue using GTX85253 KDM3A antibody.
Working concentration : 20 μg/ml

Anti-KDM3A antibody used in IHC (Paraffin sections) (IHC-P). GTX85253

GTX85253 IHC-P Image

IHC-P analysis of rat liver tissue using GTX85253 KDM3A antibody.
Working concentration : 5 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399