KDM3B antibody [C2C3], C-term

Name: KDM3B antibody [C2C3], C-term
Brand: GeneTex
SKU: GTX116198
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-KDM3B antibody [C2C3], C-term used in Western Blot (WB). GTX116198

Anti-KDM3B antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX116198

Cat. No. GTX116198

Reference 1

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IP
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

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View product citations for antibody GTX116198 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IP	1:100-1:500

Not tested in other applications.

Calculated MW

192 kDa. ( Note )

Product Note

Knockdown/Knockout validation was supported by references (PMID:36077010)

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human KDM3B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10621774

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lysine demethylase 3B , 5qNCA , C5orf7 , JMJD1B , NET22

Background

Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Demethylation of Lys residue generates formaldehyde and succinate. May have tumor suppressor activity.

Database

Gene ID: 51780 KDM3B
UniProt: Q7LBC6 KDM3B

Research Area

DATA IMAGES

GTX116198 WB Image

NT2D1 whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with KDM3B antibody [C2C3], C-term (GTX116198) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX116198 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with KDM3B antibody [C2C3], C-term (GTX116198) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX116198 IP Image

KDM3B antibody [C2C3], C-term immunoprecipitates KDM3B protein in IP experiments.
IP samples: NT2D1 whole cell extract
A. 50 μg NT2D1 whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of KDM3B protein by 4 μg KDM3B antibody [C2C3], C-term (GTX116198)
5 % SDS-PAGE
The immunoprecipitated KDM3B protein was detected by KDM3B antibody [C2C3], C-term (GTX116198) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

REFERENCE

Application Reference

D'Amore C et al. Int J Mol Sci 2022; 23 (17) KDM2A and KDM3B as Potential Targets for the Rescue of F508del-CFTR.

Application : WB

Reactivity : Human

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REVIEW