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KDM5A / Jarid1A / RBBP2 antibody [18E8]

KO/KD
Anti-KDM5A / Jarid1A / RBBP2 antibody [18E8] used in Western Blot (WB). GTX00688
Anti-KDM5A / Jarid1A / RBBP2 antibody [18E8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00688

Cat. No. GTX00688

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 18E8

Isotype

 IgG2a

Application

 WB, ICC/IF, FACS

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all KDM5A / Jarid1A / RBBP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB ~1 μg/ml
ICC/IF ~1 μg/ml
FACS using 1 μg for 10⁶ cells.
Not tested in other applications.

Calculated MW

192 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to human RBP2, amino acids 1416-1434.

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lysine demethylase 5A , RBBP-2 , RBBP2 , RBP2

Cellular Localization

Nucleus,Nucleolus

Background

This gene encodes a member of the Jumonji, AT-rich interactive domain 1 (JARID1) histone demethylase protein family. The encoded protein plays a role in gene regulation through the histone code by specifically demethylating lysine 4 of histone H3. The encoded protein interacts with many other proteins, including retinoblastoma protein, and is implicated in the transcriptional regulation of Hox genes and cytokines. This gene may play a role in tumor progression. [provided by RefSeq, Aug 2013]

Database

Research Area

DATA IMAGES

Anti-KDM5A / Jarid1A / RBBP2 antibody [18E8] used in Western Blot (WB). GTX00688

GTX00688 WB Image

KO/KD

WB analysis of various samples using GTX00688 KDM5A / Jarid1A / RBBP2 antibody [18E8].
Left panel : SDS-PAGE with CBB staining
Right panel : Primary antibody
Lane 1 : HeLa cells transfected control siRNA
Lane 2 : HeLa cells transfected RBP2 siRNA
Lane 3 : MCF-7 whole cell lysate
Lane 4 : U2OS whole cell lysate
Lane 5 : NIH3T3 whole cell lysate
Lane 6 : J1 (mouse ES cells) whole cell lysate

Anti-KDM5A / Jarid1A / RBBP2 antibody [18E8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00688

GTX00688 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX00688 KDM5A / Jarid1A / RBBP2 antibody [18E8].
Dilution : 1:2000
Fixation : 4% PFA, overnight
Permeabilization : 0.25% Triton X-100 in PBS for 10 min

REFERENCE

Clone Reference

Nishibuchi G et al. J Biol Chem.? 2014; 289 (42):28956-70 Physical and functional interactions between the histone H3K4 demethylase KDM5A and the nucleosome remodeling and deacetylase (NuRD) complex.
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 399