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KDM5A / Jarid1A / RBBP2 antibody [18E8]

Anti-KDM5A / Jarid1A / RBBP2 antibody [18E8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00688
Anti-KDM5A / Jarid1A / RBBP2 antibody [18E8] used in Western Blot (WB). GTX00688

Cat No. GTX00688

Host

Mouse

Clonality

Monoclonal

Clone Name

18E8

Isotype

IgG2a

Application

WB, ICC/IF, FACS

Reactivity

Human, Mouse
Package
100 μg ($359)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB ~1 μg/ml
ICC/IF ~1 μg/ml
FACS using 1 μg for 10⁶ cells.
Not tested in other applications.

Calculated MW

192 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to human RBP2, amino acids 1416-1434.

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

lysine demethylase 5A , RBBP-2 , RBBP2 , RBP2

Cellular Localization

Nucleus,Nucleolus

Background

This gene encodes a member of the Jumonji, AT-rich interactive domain 1 (JARID1) histone demethylase protein family. The encoded protein plays a role in gene regulation through the histone code by specifically demethylating lysine 4 of histone H3. The encoded protein interacts with many other proteins, including retinoblastoma protein, and is implicated in the transcriptional regulation of Hox genes and cytokines. This gene may play a role in tumor progression. [provided by RefSeq, Aug 2013]

Database

Research Area

DATA IMAGES

Anti-KDM5A / Jarid1A / RBBP2 antibody [18E8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00688

GTX00688 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX00688 KDM5A / Jarid1A / RBBP2 antibody [18E8].
Dilution : 1:2000
Fixation : 4% PFA, overnight
Permeabilization : 0.25% Triton X-100 in PBS for 10 min

Anti-KDM5A / Jarid1A / RBBP2 antibody [18E8] used in Western Blot (WB). GTX00688

GTX00688 WB Image

WB analysis of various samples using GTX00688 KDM5A / Jarid1A / RBBP2 antibody [18E8].
Left panel : SDS-PAGE with CBB staining
Right panel : Primary antibody
Lane 1 : HeLa cells transfected control siRNA
Lane 2 : HeLa cells transfected RBP2 siRNA
Lane 3 : MCF-7 whole cell lysate
Lane 4 : U2OS whole cell lysate
Lane 5 : NIH3T3 whole cell lysate
Lane 6 : J1 (mouse ES cells) whole cell lysate

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf