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KDM5D antibody

Orthogonal
Anti-KDM5D antibody used in Western Blot (WB). GTX129826

Cat No. GTX129826

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($329),
25 μl ($169)
See all KDM5D products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

174 kDa. ( Note )

Positive Control

Jurkat nuclear extract

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human KDM5D. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886099

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

lysine demethylase 5D , HY , HYA , JARID1D , SMCY

Cellular Localization

Nucleus

Background

This gene encodes a protein containing zinc finger domains. A short peptide derived from this protein is a minor histocompatibility antigen which can lead to graft rejection of male donor cells in a female recipient. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-KDM5D antibody used in Western Blot (WB). GTX129826

GTX129826 WB Image

Orthogonal

Jurkat whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with KDM5D antibody (GTX129826) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169