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KDM6A antibody [HL1731]

Recombinant antibody KO/KD Orthogonal
Anti-KDM6A antibody [HL1731] used in Western Blot (WB). GTX637379
Anti-KDM6A antibody [HL1731] used in Western Blot (WB). GTX637379
Anti-KDM6A antibody [HL1731] used in IHC (Paraffin sections) (IHC-P). GTX637379
Anti-KDM6A antibody [HL1731] used in IHC (Paraffin sections) (IHC-P). GTX637379
Anti-KDM6A antibody [HL1731] used in IHC (Paraffin sections) (IHC-P). GTX637379
Anti-KDM6A antibody [HL1731] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637379
Anti-KDM6A antibody [HL1731] used in Western Blot (WB). GTX637379
Anti-KDM6A antibody [HL1731] used in Western Blot (WB). GTX637379

Cat. No. GTX637379

Reference 1

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1731

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Cat, Dog
Package
100 μl ($399),
25 μl ($169)
See all KDM6A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

154 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human KDM6A. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lysine demethylase 6A , KABUK2 , UTX , bA386N14.2

Cellular Localization

Nucleus

Background

This gene is located on the X chromosome and is the corresponding locus to a Y-linked gene which encodes a tetratricopeptide repeat (TPR) protein. The encoded protein of this gene contains a JmjC-domain and catalyzes the demethylation of tri/dimethylated histone H3. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Apr 2014]

Database

Research Area

DATA IMAGES

Anti-KDM6A antibody [HL1731] used in Western Blot (WB). GTX637379

GTX637379 WB Image

KO/KD

Non-transfected (–) and transfected (+) SK-N-SH whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with KDM6A antibody [HL1731] (GTX637379) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-KDM6A antibody [HL1731] used in Western Blot (WB). GTX637379

GTX637379 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with KDM6A antibody [HL1731] (GTX637379) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-KDM6A antibody [HL1731] used in IHC (Paraffin sections) (IHC-P). GTX637379

GTX637379 IHC-P Image

KDM6A antibody [HL1731] detects KDM6A protein by immunohistochemical analysis.
Sample: Paraffin-embedded dog brain.
KDM6A stained by KDM6A antibody [HL1731] (GTX637379) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-KDM6A antibody [HL1731] used in IHC (Paraffin sections) (IHC-P). GTX637379

GTX637379 IHC-P Image

KDM6A antibody [HL1731] detects KDM6A protein by immunohistochemical analysis.
Sample: Paraffin-embedded cat tissue.
KDM6A stained by KDM6A antibody [HL1731] (GTX637379) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-KDM6A antibody [HL1731] used in IHC (Paraffin sections) (IHC-P). GTX637379

GTX637379 IHC-P Image

KDM6A antibody [HL1731] detects KDM6A protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
KDM6A stained by KDM6A antibody [HL1731] (GTX637379) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-KDM6A antibody [HL1731] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637379

GTX637379 ICC/IF Image

KDM6A antibody [HL1731] detects KDM6A protein by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: KDM6A stained by KDM6A antibody [HL1731] (GTX637379) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Anti-KDM6A antibody [HL1731] used in Western Blot (WB). GTX637379

GTX637379 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with KDM6A antibody [HL1731] (GTX637379) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-KDM6A antibody [HL1731] used in Western Blot (WB). GTX637379

GTX637379 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with KDM6A antibody [HL1731] (GTX637379) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

Application Reference

Zhao Y et al. Cell Stress Chaperones 2023; 28 (4) : 375-384 ROS inhibition increases KDM6A-mediated NOX2 transcription and promotes macrophages oxidative stress and M1 polarization.
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Package List Price ($)
$ 399
$ 169
Custom Conjugations