Non-transfected (–) and transfected (+) SK-N-SH whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with KDM6A antibody [HL1731] (GTX637379) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

KDM6A antibody [HL1731] detects KDM6A protein by immunohistochemical analysis.
Sample: Paraffin-embedded cat tissue.
KDM6A stained by KDM6A antibody [HL1731] (GTX637379) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

KDM6A antibody [HL1731] detects KDM6A protein by immunohistochemical analysis.
Sample: Paraffin-embedded dog brain.
KDM6A stained by KDM6A antibody [HL1731] (GTX637379) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

KDM6A antibody [HL1731] detects KDM6A protein by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: KDM6A stained by KDM6A antibody [HL1731] (GTX637379) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

KDM6A antibody [HL1731] detects KDM6A protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
KDM6A stained by KDM6A antibody [HL1731] (GTX637379) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with KDM6A antibody [HL1731] (GTX637379) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with KDM6A antibody [HL1731] (GTX637379) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with KDM6A antibody [HL1731] (GTX637379) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.