KDM6A antibody [N2C1], Internal

Name: KDM6A antibody [N2C1], Internal
Brand: GeneTex
SKU: GTX121246
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-KDM6A antibody [N2C1], Internal used in ChIP assay (ChIP assay). GTX121246

Anti-KDM6A antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX121246

Anti-KDM6A antibody [N2C1], Internal used in Western Blot (WB). GTX121246

Anti-KDM6A antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121246

Anti-KDM6A antibody [N2C1], Internal used in Immunoprecipitation (IP). GTX121246

Cat. No. GTX121246

Reference 8

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP, ChIP assay
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all KDM6A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	1:100-1:500
ChIP assay	Assay dependent

Not tested in other applications.

Calculated MW

154 kDa. ( Note )

Positive Control

U87-MG

Predict Reactivity

Bovine, Dog, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.68 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human KDM6A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10722382

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

lysine demethylase 6A , KABUK2 , UTX , bA386N14.2

Cellular Localization

Nucleus

Background

This gene is located on the X chromosome and is the corresponding locus to a Y-linked gene which encodes a tetratricopeptide repeat (TPR) protein. The encoded protein of this gene contains a JmjC-domain and catalyzes the demethylation of tri/dimethylated histone H3. [provided by RefSeq]

Database

Gene ID: 7403 KDM6A
UniProt: O15550 KDM6A

Research Area

DATA IMAGES

GTX121246 ChIP assay Image

ChIP was performed with HeLa chromatin extract and 5 μg of either normal rabbit IgG or anti-KDM6A antibody. The precipitated DNA was detected by PCR with primer set targeting to RBL1 promoter.

GTX121246 IHC-P Image

KDM6A antibody [N2C1], Internal detects KDM6A protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
KDM6A stained by KDM6A antibody [N2C1], Internal (GTX121246) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX121246 WB Image

Non-transfected (–) and transfected (+) U87-MG whole cell extracts (60 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with KDM6A antibody [N2C1], Internal (GTX121246) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX121246 IHC-P Image

KDM6A antibody [N2C1], Internal detects KDM6A protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lymph node.
KDM6A stained by KDM6A antibody [N2C1], Internal (GTX121246) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX121246 IHC-P Image

KDM6A antibody [N2C1], Internal detects KDM6A protein at nucleus on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
KDM6A antibody [N2C1], Internal (GTX121246) dilution: 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX121246 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using KDM6A(GTX121246) antibody at 1:500 dilution.

GTX121246 IP Image

KDM6A antibody [N2C1], Internal immunoprecipitates KDM6A protein in IP experiments.
IP samples: SK-N-SH whole cell extract
A. 30 μg SK-N-SH whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of KDM6A protein by 4 μg KDM6A antibody [N2C1], Internal (GTX121246)
5 % SDS-PAGE
The immunoprecipitated KDM6A protein was detected by KDM6A antibody [N2C1], Internal (GTX121246) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

GTX121246 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H441 xenograft, using KDM6A(GTX121246) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX121246 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with KDM6A antibody [N2C1], Internal (GTX121246) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX121246 WB Image

REFERENCE

Application Reference

Kitajima S et al. Sci Rep 2021; 11 (1) : 4538 A KDM6 inhibitor potently induces ATF4 and its target gene expression through HRI activation and by UTX inhibition.

Application : WB

Reactivity : Human

Perrigue PM et al. Cancers (Basel) 2020; 12 (7) Cancer Stem Cell-Inducing Media Activates Senescence Reprogramming in Fibroblasts.

Davis EJ et al. Sci Transl Med 2020; 12 (558) A second X chromosome contributes to resilience in a mouse model of Alzheimer's disease.

Application : WB

Reactivity : Mouse

Imuta H et al. Heart Vessels 2020; Histone methylation and demethylation are implicated in the transient and sustained activation of the interleukin-1β gene in murine macrophages.

Application : WB

Reactivity : Mouse

Tang QY et al. Stem Cell Reports 2020; 15 (2) : 439-453 UTX Regulates Human Neural Differentiation and Dendritic Morphology by Resolving Bivalent Promoters.

Application : WB

Reactivity : Human

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