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KDM6A antibody [N2C1], Internal

KO/KD Orthogonal
Anti-KDM6A antibody [N2C1], Internal used in Western Blot (WB). GTX121246
Anti-KDM6A antibody [N2C1], Internal used in Western Blot (WB). GTX121246
Anti-KDM6A antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX121246
Anti-KDM6A antibody [N2C1], Internal used in Immunoprecipitation (IP). GTX121246
Anti-KDM6A antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX121246
Anti-KDM6A antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121246
Anti-KDM6A antibody [N2C1], Internal used in ChIP assay (ChIP assay). GTX121246
Anti-KDM6A antibody [N2C1], Internal used in Western Blot (WB). GTX121246
Anti-KDM6A antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX121246
Anti-KDM6A antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX121246
Anti-KDM6A antibody [N2C1], Internal used in Western Blot (WB). GTX121246

Cat. No. GTX121246

Reference 10

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, ChIP assay

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all KDM6A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

154 kDa. ( Note )

Positive Control

U87-MG

Predict Reactivity

Bovine, Dog, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.68 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human KDM6A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10722382

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lysine demethylase 6A , KABUK2 , UTX , bA386N14.2

Cellular Localization

Nucleus

Background

This gene is located on the X chromosome and is the corresponding locus to a Y-linked gene which encodes a tetratricopeptide repeat (TPR) protein. The encoded protein of this gene contains a JmjC-domain and catalyzes the demethylation of tri/dimethylated histone H3. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-KDM6A antibody [N2C1], Internal used in Western Blot (WB). GTX121246

GTX121246 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with KDM6A antibody [N2C1], Internal (GTX121246) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-KDM6A antibody [N2C1], Internal used in Western Blot (WB). GTX121246

GTX121246 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with KDM6A antibody [N2C1], Internal (GTX121246) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-KDM6A antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX121246

GTX121246 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H441 xenograft, using KDM6A(GTX121246) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-KDM6A antibody [N2C1], Internal used in Immunoprecipitation (IP). GTX121246

GTX121246 IP Image

KDM6A antibody [N2C1], Internal immunoprecipitates KDM6A protein in IP experiments.
IP samples: SK-N-SH whole cell extract
A. 30 μg SK-N-SH whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of KDM6A protein by 4 μg KDM6A antibody [N2C1], Internal (GTX121246)
5 % SDS-PAGE
The immunoprecipitated KDM6A protein was detected by KDM6A antibody [N2C1], Internal (GTX121246) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-KDM6A antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX121246

GTX121246 IHC-P Image

KDM6A antibody [N2C1], Internal detects KDM6A protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
KDM6A stained by KDM6A antibody [N2C1], Internal (GTX121246) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-KDM6A antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121246

GTX121246 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using KDM6A(GTX121246) antibody at 1:500 dilution.

Anti-KDM6A antibody [N2C1], Internal used in ChIP assay (ChIP assay). GTX121246

GTX121246 ChIP assay Image

ChIP was performed with HeLa chromatin extract and 5 μg of either normal rabbit IgG or anti-KDM6A antibody. The precipitated DNA was detected by PCR with primer set targeting to RBL1 promoter.

Anti-KDM6A antibody [N2C1], Internal used in Western Blot (WB). GTX121246

GTX121246 WB Image

KO/KD

Non-transfected (–) and transfected (+) U87-MG whole cell extracts (60 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with KDM6A antibody [N2C1], Internal (GTX121246) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-KDM6A antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX121246

GTX121246 IHC-P Image

KDM6A antibody [N2C1], Internal detects KDM6A protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lymph node.
KDM6A stained by KDM6A antibody [N2C1], Internal (GTX121246) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-KDM6A antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX121246

GTX121246 IHC-P Image

KDM6A antibody [N2C1], Internal detects KDM6A protein at nucleus on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
KDM6A antibody [N2C1], Internal (GTX121246) dilution: 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-KDM6A antibody [N2C1], Internal used in Western Blot (WB). GTX121246

GTX121246 WB Image

The data was published in the journal Nat Commun in 2017. PMID: 28387316

REFERENCE

Application Reference

Luo D et al. Exp Hematol Oncol 2023; 12 (1) : 77 CUL4B-DDB1-COP1-mediated UTX downregulation promotes colorectal cancer progression.
W Sun et al. Epigenetics 2023; 18 (1) : 2222245 Catalytic domain-dependent and -independent transcriptional activities of the tumour suppressor histone H3K27 demethylase UTX/KDM6A in specific cancer types
Application : WB
Reactivity : Human
Kitajima S et al. Sci Rep 2021; 11 (1) : 4538 A KDM6 inhibitor potently induces ATF4 and its target gene expression through HRI activation and by UTX inhibition.
Application : WB
Reactivity : Human
Perrigue PM et al. Cancers (Basel) 2020; 12 (7) Cancer Stem Cell-Inducing Media Activates Senescence Reprogramming in Fibroblasts.
Davis EJ et al. Sci Transl Med 2020; 12 (558) A second X chromosome contributes to resilience in a mouse model of Alzheimer's disease.
Application : WB
Reactivity : Mouse
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REVIEW

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Application Reference
Luo D et al. Exp Hematol Oncol 2023; 12 (1):77 CUL4B-DDB1-COP1-mediated UTX downregulation promotes colorectal cancer progression.
W Sun et al. Epigenetics 2023; 18 (1):2222245 Catalytic domain-dependent and -independent transcriptional activities of the tumour suppressor histone H3K27 demethylase UTX/KDM6A in specific cancer types
Application : WB
Reactivity : Human
Kitajima S et al. Sci Rep 2021; 11 (1):4538 A KDM6 inhibitor potently induces ATF4 and its target gene expression through HRI activation and by UTX inhibition.
Application : WB
Reactivity : Human
Perrigue PM et al. Cancers (Basel) 2020; 12 (7) Cancer Stem Cell-Inducing Media Activates Senescence Reprogramming in Fibroblasts.
Davis EJ et al. Sci Transl Med 2020; 12 (558) A second X chromosome contributes to resilience in a mouse model of Alzheimer's disease.
Application : WB
Reactivity : Mouse
Imuta H et al. Heart Vessels 2020; Histone methylation and demethylation are implicated in the transient and sustained activation of the interleukin-1β gene in murine macrophages.
Application : WB
Reactivity : Mouse
Tang QY et al. Stem Cell Reports 2020; 15 (2):439-453 UTX Regulates Human Neural Differentiation and Dendritic Morphology by Resolving Bivalent Promoters.
Application : WB
Reactivity : Human
Kato H et al. Oncogene. 2020; (Epub) Cancer-derived UTX TPR mutations G137V and D336G impair interaction with MLL3/4 complexes and affect UTX subcellular localization.
Application : WB
Reactivity : Human
Gozdecka M et al. Nat Genet 2018; (Epub) UTX-mediated enhancer and chromatin remodeling suppresses myeloid leukemogenesis through noncatalytic inverse regulation of ETS and GATA programs.
Application : WB
Reactivity : Mouse
Zhang H et al. Nat Commun 2017; 8:14922 Lkb1 inactivation drives lung cancer lineage switching governed by Polycomb Repressive Complex 2.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169