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KEAP1 antibody [1F10B6]

Anti-KEAP1 antibody [1F10B6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60660
Anti-KEAP1 antibody [1F10B6] used in Western Blot (WB). GTX60660
Anti-KEAP1 antibody [1F10B6] used in ELISA (ELISA). GTX60660
Anti-KEAP1 antibody [1F10B6] used in Flow cytometry (FACS). GTX60660

Cat. No. GTX60660

Host

Mouse

Clonality

Monoclonal

Clone Name

1F10B6

Isotype

IgG1

Application

WB, ICC/IF, FACS, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/50
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human KEAP1 (AA: 380-624) expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_2887995

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

kelch like ECH associated protein 1 , INrf2 , KLHL19

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-KEAP1 antibody [1F10B6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60660

GTX60660 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX60660 KEAP1 antibody [1F10B6].
Green : KEAP1
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-KEAP1 antibody [1F10B6] used in Western Blot (WB). GTX60660

GTX60660 WB Image

WB analysis of NIH3T3 (1), and A549 (2) cell lysate using GTX60660 KEAP1 antibody [1F10B6].

Anti-KEAP1 antibody [1F10B6] used in ELISA (ELISA). GTX60660

GTX60660 ELISA Image

ELISA analysis of antigen using GTX60660 KEAP1 antibody [1F10B6].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-KEAP1 antibody [1F10B6] used in Flow cytometry (FACS). GTX60660

GTX60660 FACS Image

FACS analysis of HepG2 cells using GTX60660 KEAP1 antibody [1F10B6].
Green : KEAP1
Purple : negative control

REFERENCE

REVIEW

KEAP1 antibody [1F10B6] Cat. No. GTX60660

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Nutrients in 2018.PMID: 30200495
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Int J Mol Sci in 2017. PMID: 28926934
SDS
Sodium Azide.pdf
Package List Price ($)
$ 399