KEAP1 antibody [1F10B6]

Name: KEAP1 antibody [1F10B6]
Brand: GeneTex
SKU: GTX60660
Price: 399.00 USD
Rating: 5 (2 reviews)

Anti-KEAP1 antibody [1F10B6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60660

Anti-KEAP1 antibody [1F10B6] used in Western Blot (WB). GTX60660

Anti-KEAP1 antibody [1F10B6] used in ELISA (ELISA). GTX60660

Anti-KEAP1 antibody [1F10B6] used in Flow cytometry (FACS). GTX60660

Cat. No. GTX60660

Reference 5

Host	Mouse
Clonality	Monoclonal
Clone Name	1F10B6
Isotype	IgG1
Application	WB, ICC/IF, FACS, ELISA
Reactivity	Human, Mouse, Rat
Package	100 μl ($399)

See all KEAP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1/500 - 1/2000
ICC/IF	1/50
FACS	1/200 - 1/400
ELISA	1/10000

Not tested in other applications.

Calculated MW

70 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human KEAP1 (AA: 380-624) expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_2887995

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

kelch like ECH associated protein 1 , INrf2 , KLHL19

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Gene ID: 9817 KEAP1
UniProt: Q14145 KEAP1

Research Area

DATA IMAGES

GTX60660 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX60660 KEAP1 antibody [1F10B6].
Green : KEAP1
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

GTX60660 WB Image

WB analysis of NIH3T3 (1), and A549 (2) cell lysate using GTX60660 KEAP1 antibody [1F10B6].

GTX60660 ELISA Image

ELISA analysis of antigen using GTX60660 KEAP1 antibody [1F10B6].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

GTX60660 FACS Image

FACS analysis of HepG2 cells using GTX60660 KEAP1 antibody [1F10B6].
Green : KEAP1
Purple : negative control

REFERENCE

Application Reference

Liang Xia et al. Oxid Med Cell Longev 2022; 2022 : 9925919 Apatinib Induces Ferroptosis of Glioma Cells through Modulation of the VEGFR2/Nrf2 Pathway.

Application : WB

Reactivity : Human

Lin Li et al. Oxid Med Cell Longev 2022; 2022 : 4619760 Protective Effects of Cannabidiol on Chemotherapy-Induced Oral Mucositis via the Nrf2/Keap1/ARE Signaling Pathways.

Application : WB

Reactivity : Human

Arellano-Buend?a AS et al. Antioxidants (Basel) 2020; 9 (11) Effects of Allicin on Pathophysiological Mechanisms during the Progression of Nephropathy Associated to Diabetes.

Application : WB

Reactivity : Rat

Hsieh YH et al. Nutrients 2018; 10 (9) Ginsenoside Rh2 Ameliorates Lipopolysaccharide-Induced Acute Lung Injury by Regulating the TLR4/PI3K/Akt/mTOR, Raf-1/MEK/ERK, and Keap1/Nrf2/HO-1 Signaling Pathways in Mice.

Garcia Trejo EMA et al. Int J Mol Sci 2017; 18 (9) The Beneficial Effects of Allicin in Chronic Kidney Disease Are Comparable to Losartan.

Application : WB

Reactivity : Rat

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