GeneTex
United States (US)

KEAP1 antibody [C1C3]

GTX106142_WB_19011506_545.jpg
GTX106142_39897_42403_IFA_19011506_894.jpg

Cat No. GTX106142

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

NT2D1 , IMR32 , MCF-7

Predict Reactivity

Mouse, Rat, Bovine, Pig, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human KEAP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

INrf2 antibody, KIAA0132 antibody, KLHL19 antibody, MGC10630 antibody, MGC1114 antibody, MGC20887 antibody, MGC4407 antibody, MGC9454 antibody, KEAP1 antibody, cytosolic inhibitor of Nrf2 antibody, kelch-like ECH-associated protein 1 antibody, kelch-like protein 19 antibody

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX106142_WB_19011506_545.jpg

GTX106142 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
7.5% SDS PAGE
GTX106142 diluted at 1:1000

GTX106142_39897_42403_IFA_19011506_894.jpg

GTX106142 ICC/IF Image

KEAP1 antibody [C1C3] detects KEAP1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: KEAP1 protein stained by KEAP1 antibody [C1C3] (GTX106142) diluted at 1:500.
Blue: Hoechst 33342 staining.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169