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KEAP1 antibody

Anti-KEAP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54329
Anti-KEAP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54329
Anti-KEAP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54329
Anti-KEAP1 antibody used in Western Blot (WB). GTX54329

Cat. No. GTX54329

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all KEAP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 325-624 of human KEAP1 (NP_036421.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887852

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

kelch like ECH associated protein 1 , INrf2 , KLHL19

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-KEAP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54329

GTX54329 ICC/IF Image

ICC/IF analysis of C6 cells using GTX54329 KEAP1 antibody.
Blue : DAPI
Dilution : 1:100

Anti-KEAP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54329

GTX54329 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX54329 KEAP1 antibody.
Blue : DAPI
Dilution : 1:100

Anti-KEAP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54329

GTX54329 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX54329 KEAP1 antibody.
Dilution : 1:100

Anti-KEAP1 antibody used in Western Blot (WB). GTX54329

GTX54329 WB Image

WB analysis of various sample lysates using GTX54329 KEAP1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

Application Reference

Wu CT et al. Oxid Med Cell Longev 2019; 2019 : 9056845 Salvianolic Acid C against Acetaminophen-Induced Acute Liver Injury by Attenuating Inflammation, Oxidative Stress, and Apoptosis through Inhibition of the Keap1/Nrf2/HO-1 Signaling.
Application : WB
Reactivity : Mouse
Lee J et al. Redox Biol 2018; 18 : 43266 Neuroprotection against 6-OHDA toxicity in PC12 cells and mice through the Nrf2 pathway by a sesquiterpenoid from Tussilago farfara.
Application : WB
Reactivity : Rat
Lin WC et al. RSC Adv. 2017; 7 : 21234-21251 Anti-inflammatory activity of Sanghuangporus sanghuang by suppressing the TLR4-mediated PI3K/AKT/mTOR/IKKβ signaling pathway.
Application : WB
Reactivity : Mouse
Tapia E et al. Free Radic Res 2016; 50 (7) : 781-92 Mycophenolate mofetil and curcumin provide comparable therapeutic benefit in experimental chronic kidney disease: role of Nrf2-Keap1 and renal * pathways.
Application : WB
Reactivity : Rat
Lee JC et al. Br J Pharmacol 2014; 171 (1) : 237-52 Andrographolide exerts anti-hepatitis C virus activity by up-regulating haeme oxygenase-1 via the p38 MAPK/Nrf2 pathway in human hepatoma cells.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399