GeneTex
United States (US)

KGF antibody

Cat No. GTX10423

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA, Neut
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.2 μg/ml
IHC-P 15 μg/ml
ELISA 0.5-1 μg/ml
Neut ~6-12 μg/ml
Not tested in other applications.

Calculated MW

23 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 5% trehalose

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

recombinant human keratinocyte growth factor (FGF-7) expressed in E. coli.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Fibroblast Growth Factor 7,Hbgf-7,Kgf,Fgf7

Background

Keratinocyte Growth Factor (KGF, FGF7) was originally isolated from the conditioned medium of a human embryonic lung fibroblast cell line as a mitogen specific for epithelial cells. The transcript for KGF is detected in stromal but not epithelial cells. It has been suggested that KGF is a mesenchymal cell-derived paracrine growth factor that stimulates epithelial cell growth. The N-terminal residues (31 amino acid residues) of KGF are cleaved from the KGF precursor protein (194 amino acid residues) to generate the mature form of KGF (163 amino acid residues). Human KGF shows species cross-reactivity and is active on mouse, monkey, and porcine cells. Mature human and mouse FGF7 have 96% amino acid sequence identity, while human and rat FGF7 have 92% amino acid sequence identity. KGF is expressed in a number of stromal fibroblast cell lines but is absent from normal glial cells and epithelial cell lines. KGF released from stromal and dermal fibroblasts stimulates proliferation and differentiation keratinocytes and other cells in the epithelium. Adult cells known to express FGF7 include fibroblasts, smooth muscle cells, and ovarian theca cells. KGF is expressed at many stages of development throughout the mesenchyme.

Database

Package List Price ($)
$ 289