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KHSRP antibody

Anti-KHSRP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131804
Anti-KHSRP antibody used in Western Blot (WB). GTX131804
Anti-KHSRP antibody used in IHC (Paraffin sections) (IHC-P). GTX131804
Anti-KHSRP antibody used in Western Blot (WB). GTX131804
Anti-KHSRP antibody used in IHC (Paraffin sections) (IHC-P). GTX131804

Cat No. GTX131804

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Positive Control

A431 , HeLa , mouse brain

Predict Reactivity

Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.76 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human KHSRP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

KH-type splicing regulatory protein,FBP2,FUBP2,KSRP,p75

Cellular Localization

Nucleus , Cytoplasm

Background

The KHSRP gene encodes a multifunctional RNA-binding protein implicated in a variety of cellular processes, including transcription, alternative pre-mRNA splicing, and mRNA localization (Min et al., 1997 [PubMed 9136930]; Gherzi et al., 2004 [PubMed 15175153]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
Anti-KHSRP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131804

GTX131804 ICC/IF Image

KHSRP antibody detects KHSRP protein at nucleus by immunofluorescent analysis.
Sample: SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: KHSRP protein stained by KHSRP antibody (GTX131804) diluted at 1:400.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-KHSRP antibody used in Western Blot (WB). GTX131804

GTX131804 WB Image

KHSRP antibody detects KHSRP protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with KHSRP antibody (GTX131804) diluted at 1:1000.

Anti-KHSRP antibody used in IHC (Paraffin sections) (IHC-P). GTX131804

GTX131804 IHC-P Image

KHSRP antibody detects KHSRP protein at cytoplasm and nucleus in rat duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded rat duodenum.
KHSRP antibody (GTX131804) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-KHSRP antibody used in Western Blot (WB). GTX131804

GTX131804 WB Image

KHSRP antibody detects KHSRP protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with KHSRP antibody (GTX131804) diluted at 1:1000.

Anti-KHSRP antibody used in IHC (Paraffin sections) (IHC-P). GTX131804

GTX131804 IHC-P Image

KHSRP antibody detects KHSRP protein at cytoplasm and nucleus in mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
KHSRP antibody (GTX131804) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169