APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
A431 , HeLa , mouse brain
Predict Reactivity
Chicken, Xenopus tropicalis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.76 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human KHSRP. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886523
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
KH-type splicing regulatory protein , FBP2 , FUBP2 , KSRP , p75
Cellular Localization
Nucleus , Cytoplasm
Background
The KHSRP gene encodes a multifunctional RNA-binding protein implicated in a variety of cellular processes, including transcription, alternative pre-mRNA splicing, and mRNA localization (Min et al., 1997 [PubMed 9136930]; Gherzi et al., 2004 [PubMed 15175153]).[supplied by OMIM]
Database
Research Area
DATA IMAGES
|
GTX131804 IHC-P Image
KHSRP antibody detects KHSRP protein at cytoplasm and nucleus in mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
KHSRP antibody (GTX131804) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
|
GTX131804 WB Image
KHSRP antibody detects KHSRP protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with KHSRP antibody (GTX131804) diluted at 1:1000.
|
|
|
GTX131804 ICC/IF Image
KHSRP antibody detects KHSRP protein at nucleus by immunofluorescent analysis.
Sample: SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: KHSRP protein stained by KHSRP antibody (GTX131804) diluted at 1:400.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
|
|
|
GTX131804 IHC-P Image
KHSRP antibody detects KHSRP protein at cytoplasm and nucleus in rat duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded rat duodenum.
KHSRP antibody (GTX131804) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
|
GTX131804 WB Image
KHSRP antibody detects KHSRP protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with KHSRP antibody (GTX131804) diluted at 1:1000.
|
REFERENCE
There are currently no references for KHSRP antibody (GTX131804). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for KHSRP antibody (GTX131804). Be the first to share your experience with this product.
