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KIF2A antibody

Anti-KIF2A antibody used in IHC (Frozen sections) (IHC-Fr). GTX130808
Anti-KIF2A antibody used in Western Blot (WB). GTX130808

Cat. No. GTX130808

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-Fr

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all KIF2A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

80 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Rat, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.05 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human KIF2A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886351

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

kinesin family member 2A , CDCBM3 , HK2 , KIF2

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a plus end-directed motor required for normal mitotic progression. The encoded protein is required for normal spindle activity during mitosis and is necessary for normal brain development. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES

Anti-KIF2A antibody used in IHC (Frozen sections) (IHC-Fr). GTX130808

GTX130808 IHC-Fr Image

KIF2A antibody detects KIF2A protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse mouse cerebellum.
Green: KIF2A stained by KIF2A antibody (GTX130808) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Anti-KIF2A antibody used in Western Blot (WB). GTX130808

GTX130808 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with KIF2A antibody (GTX130808) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169