GeneTex
United States (US)

KIF4A antibody [N1N2], N-term

GTX115759_40296_IHC_18121410_662.jpg
GTX115759_40296_IFA_18121410_273.jpg
GTX115759_40296_WB_18121410_787.jpg

Cat No. GTX115759

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

140 kDa. ( Note )

Positive Control

HeLa , HepG2 , HCT116 , 293T , A431

Predict Reactivity

Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human KIF4A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Kinesin Family Member 4A,Kif4,Kif4G1,Mrx100,Kif4A

Cellular Localization

Nucleus matrix

Background

Kinesins, such as KIF4A, are microtubule-based motor proteins that generate directional movement along microtubules. They are involved in many crucial cellular processes, including cell division (Zhu and Jiang, 2005 [PubMed 15625105]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX115759_40296_IHC_18121410_662.jpg

GTX115759 IHC-P Image

Immunohistochemical analysis of paraffin-embedded TOV-112D xenograft, using KIF4A(GTX115759) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115759_40296_IFA_18121410_273.jpg

GTX115759 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using KIF4A(GTX115759) antibody at 1:200 dilution.

GTX115759_40296_WB_18121410_787.jpg

GTX115759 WB Image

KIF4A antibody detects KIF4A protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with KIF4A antibody (GTX115759) diluted by 1:1000.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169