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KIF5A antibody [C3], C-term

Anti-KIF5A antibody [C3], C-term used in Western Blot (WB). GTX104875
Anti-KIF5A antibody [C3], C-term used in Western Blot (WB). GTX104875
Anti-KIF5A antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104875
Anti-KIF5A antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104875
Anti-KIF5A antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104875

Cat. No. GTX104875

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all KIF5A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

117 kDa. ( Note )

Positive Control

Mouse brain , IMR32

Predict Reactivity

Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human KIF5A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241009

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

kinesin family member 5A , ALS25 , D12S1889 , MY050 , NEIMY , NKHC , SPG10

Cellular Localization

Cytoplasm , perinuclear region

Background

This gene encodes a member of the kinesin family of proteins. Members of this family are part of a multisubunit complex that functions as a microtubule motor in intracellular organelle transport. Mutations in this gene cause autosomal dominant spastic paraplegia 10. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-KIF5A antibody [C3], C-term used in Western Blot (WB). GTX104875

GTX104875 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with KIF5A antibody [C3], C-term (GTX104875) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-KIF5A antibody [C3], C-term used in Western Blot (WB). GTX104875

GTX104875 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with KIF5A antibody [C3], C-term (GTX104875) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-KIF5A antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104875

GTX104875 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Kinesin 5A(GTX104875) antibody at 1:500 dilution.

Anti-KIF5A antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104875

GTX104875 IHC-P Image

Kinesin 5A antibody [C3], C-term detects Kinesin 5A protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Kinesin 5A antibody [C3], C-term (GTX104875) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-KIF5A antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104875

GTX104875 IHC-P Image

Kinesin 5A antibody [C3], C-term detects Kinesin 5A protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Kinesin 5A antibody [C3], C-term (GTX104875) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169