Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with KIF6 antibody (GTX134401) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with KIF6 antibody (GTX134401) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.