*Optimal dilutions/concentrations should be determined by the researcher.
Use 10μl of the suggested working dilution to label 106 cells in 100μl.
Not tested in other applications.
This antibody also recognises a 50kDa molecule in some NK clones, which is highly homologous to p58.2 in the extracellular domain, but has a shorter cytoplasmic tail (Moretta et al. 1985).
PBS, 0.09% Sodium Azide, 0.5% BSA
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1.0 mg/ml (Please refer to the vial label for the specific concentration.)
NK cell clone E57 (Moretta et al. 1985).
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Killer Cell Immunoglobulin Like Receptor, Two Ig Domains And Long Cytoplasmic Tail 3 , Cd158B2 , Cd158B , Gl183 , Kir-023Gb , Kir-K7B , Kir-K7C , Kir2Dl , Kir2Ds5 , Kircl23 , Nkat , Nkat2 , Nkat2A , Nkat2B , P58 , Kir2Dl3
Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. The KIR genes are polymorphic and highly homologous and they are found in a cluster on chromosome 19q13.4 within the 1 Mb leukocyte receptor complex (LRC). The gene content of the KIR gene cluster varies among haplotypes, although several "framework" genes are found in all haplotypes (KIR3DL3, KIR3DP1, KIR3DL4, KIR3DL2). The KIR proteins are classified by the number of extracellular immunoglobulin domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. KIR proteins with the long cytoplasmic domain transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM), while KIR proteins with the short cytoplasmic domain lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals. The ligands for several KIR proteins are subsets of HLA class I molecules; thus, KIR proteins are thought to play an important role in regulation of the immune response. [provided by RefSeq, Jul 2008]