APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1 - 2 μg/mL |
5 μg/mL |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Mouse Kidney Tissue Lysate(GTX27936)
Product Note
KIRREL3 antibody is human, mouse and rat reactive. At least two isoforms are known to exist; this antibody will detect both isoforms. KIRREL3 antibody is predicted to not cross-react with other members of the KIRREL protein family.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
KIRREL3 antibody was raised against a 19 amino acid peptide near the amino terminus of human KIRREL3.The immunogen is located within amino acids 20 - 70 of KIRREL3.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
kirre like nephrin family adhesion molecule 3 , KIRRE , MRD4 , NEPH2 , PRO4502
Cellular Localization
Cell membrane
Background
The protein encoded by this gene is a member of the nephrin-like protein family. These proteins are expressed in fetal and adult brain, and also in podocytes of kidney glomeruli. The cytoplasmic domains of these proteins interact with the C-terminus of podocin, also expressed in the podocytes, cells involved in ensuring size- and charge-selective ultrafiltration. Mutations in this gene are associated with mental retardation autosomal dominant type 4 (MRD4). Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]
Database
Research Area
DATA IMAGES
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GTX32140 WB Image
WB analysis of mouse kidney tissue lysate using GTX32140 KIRREL3 antibody. Working concentration : (A) 1 and (B) 2 μg/ml
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GTX32140 IHC-P Image
IHC-P analysis of mouse kidney tissue using GTX32140 KIRREL3 antibody. Working concentration : 20 μg/ml
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GTX32140 IHC-P Image
IHC-P analysis of mouse kidney tissue using GTX32140 KIRREL3 antibody. Working concentration : 5 μg/ml
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REFERENCE
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REVIEW
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