GeneTex
  • Country / Location Selection

United States (US)

KLB antibody [N1N3]

Cat. No. GTX122197

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Mouse
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
Not tested in other applications.

Calculated MW

120 kDa. ( Note )

Predict Reactivity

Human, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human KLB. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11168424

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

klotho beta , BKL

Cellular Localization

Cell membrane; Single-pass type III membrane protein

Background

Contributes to the transcriptional repression of cholesterol 7-alpha-hydroxylase (CYP7A1), the rate-limiting enzyme in bile acid synthesis. Probably inactive as a glycosidase. Increases the ability of FGFR1 and FGFR4 to bind FGF21.

Database

Research Area

REFERENCE

REVIEW

KLB antibody [N1N3] Cat. No. GTX122197

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 23-May-2016
Score :
Application Tested : WB
Sample Species : Ms
Sample : 3T3-L1 cells
Amount used : 60μg
Blocking : 5% skim milk, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Figure Note : Although only sharing 77% identity with mouse KLB, GTX122197 is expected to cross-react with mouse due to highly conserved regions. Please refer to PMID: 17452648 for additional information on the 3T3-L1 cell differentiation conditions.
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169