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KLF10 antibody

Anti-KLF10 antibody used in Immunoprecipitation (IP). GTX108661
Anti-KLF10 antibody used in Western Blot (WB). GTX108661
Anti-KLF10 antibody used in Western Blot (WB). GTX108661

Cat No. GTX108661

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP, ChIP assay

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

HeLa nuclear , eGFP-human KLF10-transfected 293T

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human KLF10/TIEG1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950622

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Kruppel like factor 10 , EGR-alpha , EGRA , TIEG , TIEG1

Cellular Localization

Nucleus

Database

DATA IMAGES

Anti-KLF10 antibody used in Immunoprecipitation (IP). GTX108661

GTX108661 IP Image

KLF10 antibody immunoprecipitates KLF10 protein in IP experiments.
IP Sample: HeLa whole cell lysate/extract
A. Control with 2 μg of preimmune rabbit IgG
B. Immunoprecipitation of KLF10 protein by 2 μg of KLF10 antibody (GTX108661) 7.5% SDS-PAGE
The immunoprecipitated KLF10 protein was detected by KLF10 antibody (GTX108661) diluted at 1:1000.
EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-KLF10 antibody used in Western Blot (WB). GTX108661

GTX108661 WB Image

KLF10 antibody detects KLF10 protein by western blot analysis.
A. 30 μg HeLa whole cell lysate/extract
B. 30 μg HeLa nuclear lysate/extract
10% SDS-PAGE
KLF10 antibody (GTX108661) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-KLF10 antibody used in Western Blot (WB). GTX108661

GTX108661 WB Image

KLF10 antibody detects KLF10 protein by western blot analysis.
A. 30 μg 293T
B. 30 μg of eGFP-human KLF10-transfected 293T cells
7.5% SDS-PAGE
KLF10 antibody (GTX108661) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169