GeneTex
United States (US)

KLF10 antibody

GTX108661_39904_IP_19011507_113.jpg
GTX108661_40212_WB_Fraction_19011507_860.jpg
GTX108661_40212_WB_B_19011507_851.jpg

Cat No. GTX108661

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP, ChIP assay
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

HeLa nuclear , eGFP-human KLF10-transfected 293T

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human KLF10/TIEG1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Kruppel Like Factor 10,Egr-Alpha,Egra,Tieg,Tieg1,Klf10

Cellular Localization

Nucleus

Database

DATA IMAGES
GTX108661_39904_IP_19011507_113.jpg

GTX108661 IP Image

KLF10 antibody immunoprecipitates KLF10 protein in IP experiments.
IP Sample: HeLa whole cell lysate/extract
A. Control with 2 μg of preimmune rabbit IgG
B. Immunoprecipitation of KLF10 protein by 2 μg of KLF10 antibody (GTX108661) 7.5% SDS-PAGE
The immunoprecipitated KLF10 protein was detected by KLF10 antibody (GTX108661) diluted at 1:1000.
EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX108661_40212_WB_Fraction_19011507_860.jpg

GTX108661 WB Image

KLF10 antibody detects KLF10 protein by western blot analysis.
A. 30 µg HeLa whole cell lysate/extract
B. 30 µg HeLa nuclear lysate/extract
10% SDS-PAGE
KLF10 antibody (GTX108661) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108661_40212_WB_B_19011507_851.jpg

GTX108661 WB Image

KLF10 antibody detects KLF10 protein by western blot analysis.
A. 30 µg 293T
B. 30 µg of eGFP-human KLF10-transfected 293T cells
7.5% SDS-PAGE
KLF10 antibody (GTX108661) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169