GeneTex
United States (US)

KLF15 antibody [N1], N-term

Overexpression
GTX104839_39946_20150813_WB_B_19011506_918.jpg
GTX104839_WB_19011506_994.jpg

Cat No. GTX104839

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , human KLF15-transfected 293T

Predict Reactivity

Mouse, Rat, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human KLF15. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DKFZp779M1320 antibody, KKLF antibody, KLF15 antibody, kidney-enriched Kruppel-like factor antibody, Krueppel-like factor 15 antibody, kidney-enriched krueppel-like factor antibody, Kruppel-like factor 15 antibody

Cellular Localization

Nucleus

Background

Transcriptional activator. Binds to the GA element of the CLCNKA promoter.
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Database

DATA IMAGES
GTX104839_39946_20150813_WB_B_19011506_918.jpg

GTX104839 WB Image

Overexpression

KLF15 antibody [N1], N-term detects KLF15 protein by western blot analysis. Non-transfected (-), KLF4-transfected (+), KLF12-transfected (+) and KLF15-transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF15 antibody [N1], N-term (GTX104839) diluted at 1:1000.

GTX104839_WB_19011506_994.jpg

GTX104839 WB Image

Sample(30 ug whole cell lysate)
A:MOLT4 (GTX27912)
10% SDS PAGE
GTX104839 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169