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KLF4 antibody

Anti-KLF4 antibody used in Western Blot (WB). GTX101508
Anti-KLF4 antibody used in Western Blot (WB). GTX101508
Anti-KLF4 antibody used in Western Blot (WB). GTX101508
Anti-KLF4 antibody used in Western Blot (WB). GTX101508
Anti-KLF4 antibody used in Western Blot (WB). GTX101508
Anti-KLF4 antibody used in Western Blot (WB). GTX101508
Anti-KLF4 antibody used in Immunoprecipitation (IP). GTX101508
Anti-KLF4 antibody used in Flow cytometry (FACS). GTX101508
Anti-KLF4 antibody used in Western Blot (WB). GTX101508

Cat No. GTX101508

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, FACS, IP
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
FACS 1:50-1:200
IP 1:100-1:500
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Human ESC , HCT116 , HCT116 nuclear extract , NIH-3T3 , mouse ESC , Rat2 , *MDM-MB-231 , KLF4-transfected 293T

Predict Reactivity

Zebrafish, Sheep, Bovine, Cat, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.23 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human KLF4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Kruppel like factor 4 , EZF , GKLF

Cellular Localization

Nucleus

Background

Transcription factor which acts as both an activator and repressor. Binds the CACCC core sequence. Binds to multiple sites in the 5'-flanking region of its own gene and can activate its own transcription. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development.

Database

Research Area

DATA IMAGES
Anti-KLF4 antibody used in Western Blot (WB). GTX101508

GTX101508 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF4 antibody (GTX101508) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-KLF4 antibody used in Western Blot (WB). GTX101508

GTX101508 WB Image

Sample (20 µg of whole cell lysate)
A: mouse ESC
12% SDS PAGE
GTX101508 diluted at 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-KLF4 antibody used in Western Blot (WB). GTX101508

GTX101508 WB Image

Sample (30 µg of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX101508 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-KLF4 antibody used in Western Blot (WB). GTX101508

GTX101508 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with KLF4 antibody (GTX101508) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-KLF4 antibody used in Western Blot (WB). GTX101508

GTX101508 WB Image

Sample (20 µg of whole cell lysate)
A: human ESC
10% SDS PAGE
GTX101508 diluted at 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-KLF4 antibody used in Western Blot (WB). GTX101508

GTX101508 WB Image

HCT116 whole cell and nuclear extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF4 antibody (GTX101508) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-KLF4 antibody used in Immunoprecipitation (IP). GTX101508

GTX101508 IP Image

Immunoprecipitation of KLF4 protein from HeLa whole cell extracts using 5 μg of KLF4 antibody (GTX101508).
Western blot analysis was performed using KLF4 antibody (GTX101508).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-KLF4 antibody used in Flow cytometry (FACS). GTX101508

GTX101508 FACS Image

Flow cytometry on human embryonic stem cells, staining with KLF4 (GTX101508)antibody at 1:100 dilution(purple) or rabbit IgG (black).

Anti-KLF4 antibody used in Western Blot (WB). GTX101508

GTX101508 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF4 antibody (GTX101508) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

KLF4 antibody Cat No. GTX101508

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 21-Oct-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : MDA-MB-231 cells
Amount used : 20μg
Blocking : 5% skim milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169