GeneTex
United States (US)

KLF4 antibody

Orthogonal Overexpression
GTX101508_42984_20171006_WB_19011506_902.jpg
GTX101508_40408_WB_M_ESC_19011506_244.jpg
GTX101508_40408_WB_M_19011506_692.jpg
GTX101508_40408_20160414_WB_R_19011506_685.jpg
GTX101508_40408_WB_hESC_19011506_950.jpg
GTX101508_40408_20160121_WB_Fraction_19011506_221.jpg
GTX101508_40408_IP_19011506_323.jpg
GTX101508_40408_CT_FACS_19011506_650.jpg
GTX101508_40408_20180727_WB_B_19011506_367.jpg

Cat No. GTX101508

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, FACS, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
FACS 1:50-1:200
IP 1:100-1:500
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Human ESC , HCT116 , HCT116 nuclear extract , NIH-3T3 , mouse ESC , Rat2 , *MDM-MB-231 , KLF4-transfected 293T

Predict Reactivity

Zebrafish, Sheep, Bovine, Cat, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.23mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human KLF4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

EZF antibody, GKLF antibody, KLF4 antibody, endothelial Kruppel-like zinc finger protein antibody, Krueppel-like factor 4 antibody, gut-enriched krueppel-like factor antibody, epithelial zinc finger protein EZF antibody, Kruppel-like factor 4 (gut) antibody

Cellular Localization

Nucleus

Background

Transcription factor which acts as both an activator and repressor. Binds the CACCC core sequence. Binds to multiple sites in the 5'-flanking region of its own gene and can activate its own transcription. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development.

Database

Research Area

DATA IMAGES
GTX101508_42984_20171006_WB_19011506_902.jpg

GTX101508 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF4 antibody (GTX101508) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101508_40408_WB_M_ESC_19011506_244.jpg

GTX101508 WB Image

Sample (20 µg of whole cell lysate)
A: mouse ESC
12% SDS PAGE
GTX101508 diluted at 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101508_40408_WB_M_19011506_692.jpg

GTX101508 WB Image

Sample (30 µg of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX101508 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101508_40408_20160414_WB_R_19011506_685.jpg

GTX101508 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with KLF4 antibody (GTX101508) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101508_40408_WB_hESC_19011506_950.jpg

GTX101508 WB Image

Sample (20 µg of whole cell lysate)
A: human ESC
10% SDS PAGE
GTX101508 diluted at 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101508_40408_20160121_WB_Fraction_19011506_221.jpg

GTX101508 WB Image

Orthogonal

HCT116 whole cell and nuclear extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF4 antibody (GTX101508) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101508_40408_IP_19011506_323.jpg

GTX101508 IP Image

Immunoprecipitation of KLF4 protein from HeLa whole cell extracts using 5 μg of KLF4 antibody (GTX101508).
Western blot analysis was performed using KLF4 antibody (GTX101508).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX101508_40408_CT_FACS_19011506_650.jpg

GTX101508 FACS Image

Flow cytometry on human embryonic stem cells, staining with KLF4 (GTX101508)antibody at 1:100 dilution(purple) or rabbit IgG (black).

GTX101508_40408_20180727_WB_B_19011506_367.jpg

GTX101508 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF4 antibody (GTX101508) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

KLF4 antibody Cat No. GTX101508

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 21-Oct-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : MDA-MB-231 cells
Amount used : 20μg
Blocking : 5% skim milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169