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KLF4 antibody

KO/KD Overexpression
Anti-KLF4 antibody used in Western Blot (WB). GTX101509
Anti-KLF4 antibody used in IHC (Paraffin sections) (IHC-P). GTX101509
Anti-KLF4 antibody used in IHC (Paraffin sections) (IHC-P). GTX101509
Anti-KLF4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101509
Anti-KLF4 antibody used in Western Blot (WB). GTX101509

Cat. No. GTX101509

Reference 7
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all KLF4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

HeLa , KLF4-transfected 293T

Predict Reactivity

Rat, Sheep, Bovine, Cat, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human KLF4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11173878

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Kruppel like factor 4 , EZF , GKLF

Cellular Localization

Nucleus

Background

Transcription factor which acts as both an activator and repressor. Binds the CACCC core sequence. Binds to multiple sites in the 5'-flanking region of its own gene and can activate its own transcription. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development.

Database

Research Area

DATA IMAGES

Anti-KLF4 antibody used in Western Blot (WB). GTX101509

GTX101509 WB Image

KO/KD

Wild-type (WT) and KLF4 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with KLF4 antibody (GTX101509) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-KLF4 antibody used in IHC (Paraffin sections) (IHC-P). GTX101509

GTX101509 IHC-P Image

KLF4 antibody detects KLF4 protein at nucleus on mouse esophagus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
KLF4 antibody (GTX101509) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-KLF4 antibody used in IHC (Paraffin sections) (IHC-P). GTX101509

GTX101509 IHC-P Image

KLF4 antibody detects KLF4 protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
KLF4 antibody (GTX101509) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-KLF4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101509

GTX101509 ICC/IF Image

KLF4 antibody detects KLF4 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min.
Green: KLF4 protein stained by KLF4 antibody (GTX101509) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-KLF4 antibody used in Western Blot (WB). GTX101509

GTX101509 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF4 antibody (GTX101509) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Feng DD et al. Front Cardiovasc Med 2021; 8 : 768662 17β-Estradiol Inhibits Proliferation and Oxidative Stress in Vascular Smooth Muscle Cells by Upregulating BHLHE40 Expression.
Application : WB
Reactivity : Mouse
Zhou J et al. J Ethnopharmacol 2020; 262 : 113208 Salvia miltiorrhiza bunge exerts anti-oxidative effects through inhibiting KLF10 expression in vascular smooth muscle cells exposed to high glucose.
Application : WB
Reactivity : Mouse
Nakayama I et al. J Biochem 2020; (Epub) Regulation of Epidermal Growth Factor Receptor Expression and Morphology of Lung Epithelial Cells by Interleukin-1帣.
Application : ICC/IF
Reactivity : Human
Takeuchi Y et al. Exp Cell Res 2018; 371 (2) : 417-425 Kruppel-Like Factor 4 represses osteoblast differentiation via ciliary Hedgehog signaling.
Application : WB
Reactivity : Mouse
Moon BS et al. Nat Commun 2018; 9 (1) : 401 Kruppel-like factor 4-dependent Staufen1-mediated mRNA decay regulates cortical neurogenesis.
Reactivity : Mouse
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REVIEW

KLF4 antibody Cat. No. GTX101509

Rating
( Average 3 based on 2 users reviews)
Application
Western Blot(WB)
( Average 3 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 3 based on 1 users reviews)
Anti-KLF4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101509
Date : Anonymous submitted on 10-Apr-2019
Score :
Application Tested : ICC/IF
Sample Species : Ms
Sample : Mouse iPS cells
Blocking : 20% ImmunoBlock, 25°C, 1Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.5% Triton X-100
Primary Antibody : 1:250, 4°C, 16Hr
Anti-KLF4 antibody used in Western Blot (WB). GTX101509
Date : Anonymous submitted on 15-Jul-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells, T24 cells
Amount used : 1* 10^5 cells
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : Lane 1: A549 cells, Lane 2: T24 cells.
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169