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KLF4 antibody

Anti-KLF4 antibody used in Western Blot (WB). GTX101509
Anti-KLF4 antibody used in IHC (Paraffin sections) (IHC-P). GTX101509
Anti-KLF4 antibody used in IHC (Paraffin sections) (IHC-P). GTX101509
Anti-KLF4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101509
Anti-KLF4 antibody used in Western Blot (WB). GTX101509

Cat No. GTX101509

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Rat, Sheep, Bovine, Cat, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human KLF4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Kruppel like factor 4,EZF,GKLF

Cellular Localization

Nucleus

Background

Transcription factor which acts as both an activator and repressor. Binds the CACCC core sequence. Binds to multiple sites in the 5'-flanking region of its own gene and can activate its own transcription. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development.

Database

Research Area

DATA IMAGES
Anti-KLF4 antibody used in Western Blot (WB). GTX101509

GTX101509 WB Image

Wild-type (WT) and KLF4 knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with KLF4 antibody (GTX101509) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-KLF4 antibody used in IHC (Paraffin sections) (IHC-P). GTX101509

GTX101509 IHC-P Image

KLF4 antibody detects KLF4 protein at nucleus on mouse esophagus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
KLF4 antibody (GTX101509) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-KLF4 antibody used in IHC (Paraffin sections) (IHC-P). GTX101509

GTX101509 IHC-P Image

KLF4 antibody detects KLF4 protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
KLF4 antibody (GTX101509) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-KLF4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101509

GTX101509 ICC/IF Image

KLF4 antibody detects KLF4 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min.
Green: KLF4 protein stained by KLF4 antibody (GTX101509) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-KLF4 antibody used in Western Blot (WB). GTX101509

GTX101509 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF4 antibody (GTX101509) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

KLF4 antibody Cat No. GTX101509

Rating ( Average 3 based on 2 users reviews)
Application
Western Blot(WB) ( Average 3 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 10-Apr-2019
Score :
Application Tested : ICC/IF
Sample Species : Ms
Sample : Mouse iPS cells
Blocking : 20% ImmunoBlock, 25°C, 1Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.5% Triton X-100
Primary Antibody : 1:250, 4°C, 16Hr
Date : Anonymous submitted on 15-Jul-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells, T24 cells
Amount used : 1* 10^5 cells
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : Lane 1: A549 cells, Lane 2: T24 cells.
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169