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KLF5 antibody [HL2269]

Recombinant antibody KO/KD Orthogonal Overexpression
Anti-KLF5 antibody [HL2269] used in Western Blot (WB). GTX638321
Anti-KLF5 antibody [HL2269] used in Western Blot (WB). GTX638321
Anti-KLF5 antibody [HL2269] used in Western Blot (WB). GTX638321
Anti-KLF5 antibody [HL2269] used in IHC (Paraffin sections) (IHC-P). GTX638321
Anti-KLF5 antibody [HL2269] used in Western Blot (WB). GTX638321
Anti-KLF5 antibody [HL2269] used in IHC (Paraffin sections) (IHC-P). GTX638321
Anti-KLF5 antibody [HL2269] used in Western Blot (WB). GTX638321

Cat. No. GTX638321

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2269

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all KLF5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human KLF5.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Kruppel like factor 5 , BTEB2 , CKLF , IKLF

Cellular Localization

Nucleus

Background

This gene encodes a member of the Kruppel-like factor subfamily of zinc finger proteins. The encoded protein is a transcriptional activator that binds directly to a specific recognition motif in the promoters of target genes. This protein acts downstream of multiple different signaling pathways and is regulated by post-translational modification. It may participate in both promoting and suppressing cell proliferation. Expression of this gene may be changed in a variety of different cancers and in cardiovascular disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013]

Database

Research Area

DATA IMAGES

Anti-KLF5 antibody [HL2269] used in Western Blot (WB). GTX638321

GTX638321 WB Image

KO/KD

Wild-type (WT) and KLF5 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody [HL2269] (GTX638321) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-KLF5 antibody [HL2269] used in Western Blot (WB). GTX638321

GTX638321 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody [HL2269] (GTX638321) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-KLF5 antibody [HL2269] used in Western Blot (WB). GTX638321

GTX638321 WB Image

Whole cell extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody [HL2269] (GTX638321) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-KLF5 antibody [HL2269] used in IHC (Paraffin sections) (IHC-P). GTX638321

GTX638321 IHC-P Image

KLF5 antibody [HL2269] detects KLF5 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
KLF5 stained by KLF5 antibody [HL2269] (GTX638321) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-KLF5 antibody [HL2269] used in Western Blot (WB). GTX638321

GTX638321 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody [HL2269] (GTX638321) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-KLF5 antibody [HL2269] used in IHC (Paraffin sections) (IHC-P). GTX638321

GTX638321 IHC-P Image

KLF5 antibody [HL2269] detects KLF5 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
KLF5 stained by KLF5 antibody [HL2269] (GTX638321) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-KLF5 antibody [HL2269] used in Western Blot (WB). GTX638321

GTX638321 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody [HL2269] (GTX638321) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations