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KLHL13 antibody [8D1]

Anti-KLHL13 antibody [8D1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82815
Anti-KLHL13 antibody [8D1] used in Western Blot (WB). GTX82815
Anti-KLHL13 antibody [8D1] used in IHC (Paraffin sections) (IHC-P). GTX82815
Anti-KLHL13 antibody [8D1] used in Flow cytometry (FACS). GTX82815

Cat. No. GTX82815

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 8D1

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all KLHL13 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

74 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human KLHL13 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

kelch like family member 13 , BKLHD2

Background

This gene encodes a BTB and kelch domain containing protein and belongs to the kelch repeat domain containing superfamily of proteins. The encoded protein functions as an adaptor protein that complexes with Cullin 3 and other proteins to form the Cullin 3-based E3 ubiquitin-protein ligase complex. This complex is necessary for proper chromosome segregation and completion of cytokinesis. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]

Database

Research Area

DATA IMAGES

Anti-KLHL13 antibody [8D1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82815

GTX82815 ICC/IF Image

ICC/IF analysis of NTERA-2 cells (left) and U251 (right) cells using GTX82815 KLHL13 antibody [8D1].
Green : KLHL13
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-KLHL13 antibody [8D1] used in Western Blot (WB). GTX82815

GTX82815 WB Image

WB analysis of HeLa (1) and MCF-7 (2) cell lysate using GTX82815 KLHL13 antibody [8D1].

Anti-KLHL13 antibody [8D1] used in IHC (Paraffin sections) (IHC-P). GTX82815

GTX82815 IHC-P Image

IHC-P analysis of human brain tissue (left) and pancreas tissue (right) using GTX82815 KLHL13 antibody [8D1].

Anti-KLHL13 antibody [8D1] used in Flow cytometry (FACS). GTX82815

GTX82815 FACS Image

FACS analysis of L1 cells using GTX82815 KLHL13 antibody [8D1].
Green : KLHL13
Purple : negative control

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399