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KLHL2 antibody

Anti-KLHL2 antibody used in Western Blot (WB). GTX115614
Anti-KLHL2 antibody used in IHC (Paraffin sections) (IHC-P). GTX115614
Anti-KLHL2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115614

Cat. No. GTX115614

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all KLHL2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Positive Control

293T , A431

Predict Reactivity

Mouse, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human KLHL2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10722360

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

kelch like family member 2 , ABP-KELCH , MAV , MAYVEN

Cellular Localization

Cytoplasm , cytoskeleton

Database

Research Area

DATA IMAGES

Anti-KLHL2 antibody used in Western Blot (WB). GTX115614

GTX115614 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
7.5% SDS PAGE
GTX115614 diluted at 1:5000

Anti-KLHL2 antibody used in IHC (Paraffin sections) (IHC-P). GTX115614

GTX115614 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using KLHL2(GTX115614) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-KLHL2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115614

GTX115614 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using KLHL2(GTX115614) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169