Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using KLHL2(GTX115614) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using KLHL2(GTX115614) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
7.5% SDS PAGE
GTX115614 diluted at 1:5000