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KLHL25 antibody [C1C3]

Anti-KLHL25 antibody [C1C3] used in Western Blot (WB). GTX118140
Anti-KLHL25 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX118140
Anti-KLHL25 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118140
Anti-KLHL25 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX118140
Anti-KLHL25 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX118140
Anti-KLHL25 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX118140

Cat. No. GTX118140

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all KLHL25 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Positive Control

NT2D1

Predict Reactivity

Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.69 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human KLHL25. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619739

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

kelch like family member 25 , ENC-2 , ENC2

Database

DATA IMAGES

Anti-KLHL25 antibody [C1C3] used in Western Blot (WB). GTX118140

GTX118140 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
7.5% SDS PAGE
GTX118140 diluted at 1:1000

Anti-KLHL25 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX118140

GTX118140 IHC-P Image

Immunohistochemical analysis of paraffin-embedded mouse muscle, using KLHL25(GTX118140) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-KLHL25 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118140

GTX118140 ICC/IF Image

Immunofluorescence analysis of methanol-fixed MCF-7, using KLHL25(GTX118140) antibody at 1:200 dilution.

Anti-KLHL25 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX118140

GTX118140 IHC-P Image

KLHL25 antibody [C1C3] detects KLHL25 protein at cytosol on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
KLHL25 antibody [C1C3] (GTX118140) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-KLHL25 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX118140

GTX118140 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using KLHL25(GTX118140) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-KLHL25 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX118140

GTX118140 IHC-P Image

KLHL25 antibody [C1C3] detects KLHL25 protein at cytosol on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
KLHL25 antibody [C1C3] (GTX118140) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169