GeneTex
United States (US)

KPNA2 antibody [C3], C-term

GTX106323_39757_20141128_IHC_19011506_835.jpg
GTX106323_39757_IFA_19011506_282.jpg
GTX106323_WB_19011506_518.jpg

Cat No. GTX106323

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

293T , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

Predict Reactivity

Mouse, Rat, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.71mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human KPNA2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

IPOA1 antibody, QIP2 antibody, RCH1 antibody, SRP1alpha antibody, KPNA2 antibody, karyopherin subunit alpha-2 antibody, SRP1-alpha antibody, RAG cohort 1 antibody, importin alpha 1 antibody, importin subunit alpha-2 antibody, RAG cohort protein 1 antibody, pendulin antibody, importin-alpha-P1 antibody, importin alpha 2 antibody, "karyopherin alpha 2 (RAG cohort 1, importin alpha 1) antibody"

Cellular Localization

Cytoplasm , Nucleus

Background

The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination [provided by RefSeq]

Database

DATA IMAGES
GTX106323_39757_20141128_IHC_19011506_835.jpg

GTX106323 IHC-P Image

karyopherin alpha 2 antibody [C3], C-term detects karyopherin alpha 2 protein at nucleus on human lung adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung adenocarcinoma.
karyopherin alpha 2 antibody [C3], C-term (GTX106323) diluted at 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106323_39757_IFA_19011506_282.jpg

GTX106323 ICC/IF Image

karyopherin alpha 2 antibody [C3], C-term detects karyopherin alpha 2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: karyopherin alpha 2 protein stained by karyopherin alpha 2 antibody [C3], C-term (GTX106323) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX106323_WB_19011506_518.jpg

GTX106323 WB Image

Sample(30 µg of whole cell lysate)
A:HeLa S3(GTX14654)
B:Hep G2(GTX27900)
C:MOLT4(GTX27912)
10% SDS PAGE
GTX106323 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169