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KPNA2 antibody [C3], C-term

Anti-KPNA2 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX106323
Anti-KPNA2 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106323
Anti-KPNA2 antibody [C3], C-term used in Western Blot (WB). GTX106323

Cat. No. GTX106323

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all KPNA2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.71 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human KPNA2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950635

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

karyopherin subunit alpha 2 , IPOA1 , QIP2 , RCH1 , SRP1-alpha , SRP1alpha

Cellular Localization

Cytoplasm , Nucleus

Background

The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-KPNA2 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX106323

GTX106323 IHC-P Image

karyopherin alpha 2 antibody [C3], C-term detects karyopherin alpha 2 protein at nucleus on human lung adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung adenocarcinoma.
karyopherin alpha 2 antibody [C3], C-term (GTX106323) diluted at 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-KPNA2 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106323

GTX106323 ICC/IF Image

karyopherin alpha 2 antibody [C3], C-term detects karyopherin alpha 2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: karyopherin alpha 2 protein stained by karyopherin alpha 2 antibody [C3], C-term (GTX106323) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-KPNA2 antibody [C3], C-term used in Western Blot (WB). GTX106323

GTX106323 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with KPNA2 antibody [C3], C-term (GTX106323) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

De Munter S et al. Cell Cycle 2020; 19 (22) : 3029-3041 RepoMan stimulates the chromosome-dependent pathway of microtubule assembly.
Application : WB
Reactivity : Human
Sun CY et al. J Mol Biol 2011; 411 (2) : 397-416 Facioscapulohumeral muscular dystrophy region gene 1 is a dynamic RNA-associated and actin-bundling protein.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169