GeneTex
United States (US)

KPNA2 antibody

GTX31812_1446_IHC-P_20180221_18121410_501.jpg
GTX31812_2965_WB_20180221_18121410_856.jpg
GTX31812_2966_WB_20180221_18121410_548.jpg
GTX31812_1447_IHC-P_20180221_18121410_897.jpg

Cat No. GTX31812

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

Daudi Cell Lysate(GTX23951) , Rat Heart Tissue Lysate(GTX28912) , Human Heart Tissue Slide
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KPNA2 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human KPNA2.The immunogen is located within amino acids 40 - 90 of KPNA2.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Karyopherin Subunit Alpha 2,Ipoa1,Qip2,Rch1,Srp1-Alpha,Srp1Alpha,Kpna2

Background

The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination [provided by RefSeq, Jul 2008]

Database

DATA IMAGES
GTX31812_1446_IHC-P_20180221_18121410_501.jpg

GTX31812 IHC-P Image

IHC-P analysis of human heart tissue using GTX31812 KPNA2 antibody.
Working concentration : 5 μg/ml

GTX31812_2965_WB_20180221_18121410_856.jpg

GTX31812 WB Image

WB analysis of Daudi cell lysate using GTX31812 KPNA2 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

GTX31812_2966_WB_20180221_18121410_548.jpg

GTX31812 WB Image

WB analysis of rat heart tissue lysate using GTX31812 KPNA2 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

GTX31812_1447_IHC-P_20180221_18121410_897.jpg

GTX31812 IHC-P Image

IHC-P analysis of human heart tissue using GTX31812 KPNA2 antibody.
Working concentration : 20 μg/ml

Package List Price ($)
$ 329