APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.1-0.3μg/ml |
| Assay dependent |
| Assay dependent |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX26038-PEP).
Predict Reactivity
Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-DPTANLQTKEFNF, from the C Terminus of the protein sequence according to NP_002258.2.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
karyopherin subunit alpha 3 , IPOA4 , SRP1 , SRP1gamma , SRP4 , hSRP1
Cellular Localization
Cytoplasm , Nucleus
Background
The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC), which consists of 60-100 proteins. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion while larger molecules are transported by an active process. The protein encoded by this gene belongs to the importin alpha family, and is involved in nuclear protein import. [provided by RefSeq, Jan 2009]
Database
Research Area
DATA IMAGES
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GTX26038 ICC/IF Image
ICC/IF analysis of PFA-fixed A431 cells using GTX26038 KPNA3 antibody, C-term.
Green : Primary antibody
Blue : DAPI
Negative control: Unimmunized goat IgG (10ug/ml)
Permeabilization : 0.1% Triton X-100
Dilution : 10ug/ml
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GTX26038 FACS Image
FACS analysis of PFA-fixed A431 cells using GTX26038 KPNA3 antibody, C-term.
Blue line : Primary antibody
Black line : Negative control: Unimmunized goat IgG
Permeabilization : 0.5% Triton X-100
Dilution : 10ug/ml
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GTX26038 WB Image
WB analysis of various sample lysates using GTX26038 KPNA3 antibody, C-term.
Lane A: CaCo-2
Lane B: HEK293
Lane C: MCF-7
Loading : 35 μg
Dilution : 0.03μg/ml for A and B, 0.01μg/ml for C
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GTX26038 IHC-P Image
IHC-P analysis of human testis tissue using GTX26038 KPNA3 antibody, C-term.
Antigen retrieval : Citrate buffer pH 6.0
Dilution : 6μg/ml
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GTX26038 IHC-P Image
IHC-P analysis of human testis tissue without using GTX26038 KPNA3 antibody, C-term. (Negative control)
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REFERENCE
There are currently no references for KPNA3 antibody, C-term (GTX26038). Be the first to share your publications with this product.
REVIEW
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