GeneTex
United States (US)

KPNA4 antibody [C3], C-term

GTX106325_IHC_19011506_856.jpg
GTX106325_WB_19011506_119.jpg
GTX106325_39708_WB_M_19011506_762.jpg
GTX106325_IFA_19011506_600.jpg
GTX106325_39708_WB_R_19011506_588.jpg

Cat No. GTX106325

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

293T , HeLa , HepG2 , Molt-4 , Raji , *A549 ,

Predict Reactivity

Bovine, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Concentration

0.73mg/ml

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human KPNA4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

IPOA3 antibody, MGC12217 antibody, MGC26703 antibody, QIP1 antibody, SRP3 antibody, KPNA4 antibody, importin alpha Q1 antibody, importin subunit alpha-4 antibody, karyopherin subunit alpha-4 antibody, karyopherin alpha 4 (importin alpha 3) antibody

Background

The nuclear import of karyophilic proteins is directed by short amino acid sequences termed nuclear localization signals (NLSs). Karyopherins, or importins, are cytoplasmic proteins that recognize NLSs and dock NLS-containing proteins to the nuclear pore complex. The protein encoded by this gene shares the sequence similarity with Xenopus importin-alpha and Saccharomyces cerevisiae Srp1. This protein is found to interact with the NLSs of DNA helicase Q1 and SV40 T antigen. [provided by RefSeq]
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Database

DATA IMAGES
GTX106325_IHC_19011506_856.jpg

GTX106325 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using KPNA4(GTX106325) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106325_WB_19011506_119.jpg

GTX106325 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: HepG2(GTX27900)
C: Molt-4 (GTX27912)
7.5% SDS PAGE
GTX106325 diluted at 1:1500

GTX106325_39708_WB_M_19011506_762.jpg

GTX106325 WB Image

KPNA4 antibody [C3], C-term detects KPNA4 protein by Western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg GL261 whole cell lysate/extract
C. 30 µg C8D30 whole cell lysate/extract
D. 30 µg NIH-3T3 whole cell lysate/extract
E. 30 µg BCL-1 whole cell lysate/extract
7.5 % SDS-PAGE
KPNA4 antibody [C3], C-term (GTX106325) dilution: 1:1000

GTX106325_IFA_19011506_600.jpg

GTX106325 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using QIP1 (GTX106325) antibody at 1:200 dilution.

GTX106325_39708_WB_R_19011506_588.jpg

GTX106325 WB Image

KPNA4 antibody [C3], C-term detects KPNA4 protein by Western blot analysis.
A. 30 µg Rat2 whole cell lysate/extract
7.5 % SDS-PAGE
KPNA4 antibody [C3], C-term (GTX106325) dilution: 1:1000

Reference
REVIEW

KPNA4 antibody [C3], C-term Cat No. GTX106325

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 10-Jul-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 20μg
Blocking : 5% skim milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169