KPNA4 antibody [C3], C-term

Name: KPNA4 antibody [C3], C-term
Brand: GeneTex
SKU: GTX106325
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-KPNA4 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106325

Anti-KPNA4 antibody [C3], C-term used in Western Blot (WB). GTX106325

Anti-KPNA4 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX106325

Cat. No. GTX106325

Reference 2

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all KPNA4 products

View product citations for antibody GTX106325 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

293T , HepG2 , Molt-4 , Rat2 , Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , HeLa

Predict Reactivity

Bovine, Chicken, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.04 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human KPNA4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241016

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

karyopherin subunit alpha 4 , IPOA3 , QIP1 , SRP3

Background

The nuclear import of karyophilic proteins is directed by short amino acid sequences termed nuclear localization signals (NLSs). Karyopherins, or importins, are cytoplasmic proteins that recognize NLSs and dock NLS-containing proteins to the nuclear pore complex. The protein encoded by this gene shares the sequence similarity with Xenopus importin-alpha and Saccharomyces cerevisiae Srp1. This protein is found to interact with the NLSs of DNA helicase Q1 and SV40 T antigen. [provided by RefSeq]

Database

Gene ID: 3840 KPNA4
UniProt: O00629 KPNA4

Research Area

DATA IMAGES

GTX106325 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using QIP1 (GTX106325) antibody at 1:200 dilution.

GTX106325 WB Image

KPNA4 antibody [C3], C-term detects KPNA4 protein by Western blot analysis.
A. 30 μg Rat2 whole cell lysate/extract
7.5 % SDS-PAGE
KPNA4 antibody [C3], C-term (GTX106325) dilution: 1:1000

GTX106325 WB Image

KPNA4 antibody [C3], C-term detects KPNA4 protein by Western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
D. 30 μg NIH-3T3 whole cell lysate/extract
E. 30 μg BCL-1 whole cell lysate/extract
7.5 % SDS-PAGE
KPNA4 antibody [C3], C-term (GTX106325) dilution: 1:1000

GTX106325 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
B: Hep G2
7.5% SDS PAGE
GTX106325 diluted at 1:1000

GTX106325 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using KPNA4(GTX106325) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106325 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: HepG2(GTX27900)
C: Molt-4 (GTX27912)
7.5% SDS PAGE
GTX106325 diluted at 1:1500

GTX106325 WB Image

The data was published in the journal J Biomed Sci in 2015.PMID: 25981436

REFERENCE

Application Reference

Baker SA et al. J Biol Chem 2015; 290 (37) : 22485-93 Karyopherin α 3 and karyopherin α 4 proteins mediate the nuclear import of methyl-CpG binding protein 2.

Yang CP et al. J Biomed Sci 2015; 22 (Epub) : 33 Identification and characterization of nuclear and nucleolar localization signals in 58-kDa microspherule protein (MSP58).

Application : WB

Reactivity : Human

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