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KPNA5 antibody

Anti-KPNA5 antibody used in Western Blot (WB). GTX112203
Anti-KPNA5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112203
Anti-KPNA5 antibody used in Western Blot (WB). GTX112203

Cat. No. GTX112203

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all KPNA5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Rat, Zebrafish(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human KPNA5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11168140

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

karyopherin subunit alpha 5 , IPOA6 , SRP6

Cellular Localization

Cytoplasm

Background

The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC) which consists of 60-100 proteins and is probably 120 million daltons in molecular size. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion; larger molecules are transported by an active process. Most nuclear proteins contain short basic amino acid sequences known as nuclear localization signals (NLSs). KPNA5 protein belongs to the importin alpha protein family and is thought to be involved in NLS-dependent protein import into the nucleus. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-KPNA5 antibody used in Western Blot (WB). GTX112203

GTX112203 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
7.5% SDS PAGE
GTX112203 diluted at 1:1000

Anti-KPNA5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112203

GTX112203 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using KPNA5(GTX112203) antibody at 1:500 dilution.

Anti-KPNA5 antibody used in Western Blot (WB). GTX112203

GTX112203 WB Image

The data was published in the journal J Biomed Sci in 2015.PMID: 25981436

REFERENCE

Application Reference

Yang CP et al. J Biomed Sci 2015; 22 (Epub) : 33 Identification and characterization of nuclear and nucleolar localization signals in 58-kDa microspherule protein (MSP58).
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169