GeneTex
United States (US)

KPNA6 antibody, Internal

GTX88712_2153_WB_18122102_903.jpg
GTX88712_864_IHC-P_18122102_191.jpg

Cat No. GTX88712

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.3μg/ml
IHC-P 3-6μg/ml

Note :

IHC-P
Human Adrenal Gland shows staining of cytoplasm and some nuclear membrane (nuclear pores).

Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88712-PEP).

Predict Reactivity

Mouse, Rat, Bovine, Dog, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-STTGESVITREMVE, from the internal of the protein sequence according to NP_036448.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Karyopherin Subunit Alpha 6,Ipoa7,Kpna7,Kpna6

Background

Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. The protein encoded by this gene is a member of the importin alpha family. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES
GTX88712_2153_WB_18122102_903.jpg

GTX88712 WB Image

WB analysis of human muscle lysate using GTX88712 KPNA6 antibody, Internal.
Dilution : 0.1µg/ml
Loading : 35µg protein in RIPA buffer

GTX88712_864_IHC-P_18122102_191.jpg

GTX88712 IHC-P Image

IHC-P analysis of human adrenal gland using GTX88712 KPNA6 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8µg/ml

Package List Price ($)
$ 329