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KRR1 antibody [N1C3]

Anti-KRR1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105761
Anti-KRR1 antibody [N1C3] used in Western Blot (WB). GTX105761
Anti-KRR1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX105761

Cat. No. GTX105761

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all KRR1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

H1299 , HeLa

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human KRR1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950637

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

KRR1 small subunit processome component homolog , HRB2 , RIP-1

Cellular Localization

Nucleus , nucleolus

Background

Required for 40S ribosome biogenesis. Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly.

Database

Research Area

DATA IMAGES

Anti-KRR1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105761

GTX105761 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using KRR1(GTX105761) antibody at 1:500 dilution.

Anti-KRR1 antibody [N1C3] used in Western Blot (WB). GTX105761

GTX105761 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
B: Hela
10% SDS PAGE
GTX105761 diluted at 1:1000

Anti-KRR1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX105761

GTX105761 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SAS xenograft, using KRR1(GTX105761) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169