APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:1000-1:10000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
HepG2
Predict Reactivity
Mouse, Rat, Sheep, Bovine, Cat, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.91mg/ml(Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human KRT71. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
K6IRS1 antibody, KRT6IRS antibody, KRT6IRS1 antibody, MGC119390 antibody, MGC119391 antibody, KRT71 antibody, K71 antibody, type II inner root sheath-specific keratin-K6irs1 antibody, keratin 6 irs antibody, keratin-71 antibody, hK6irs1 antibody, "keratin, type II cytoskeletal 71 antibody", cytokeratin-71 antibody, type-II keratin Kb34 antibody, hK6irs antibody, CK-71 antibody, keratin 71 antibody
Background
K6IRS1 belongs to a family of type II keratins that are specifically expressed in the inner root sheath of hair follicles.[supplied by OMIM]
Database
DATA IMAGES
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GTX107343 ICC/IF Image
KRT71 antibody [N3C3] detects KRT71 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: KRT71 protein stained by KRT71 antibody [N3C3] (GTX107343) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
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GTX107343 IHC-P Image
KRT71 antibody [N3C3] detects KRT71 protein at membrane on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
KRT71 antibody [N3C3] (GTX107343) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX107343 WB Image
Sample (30 ug of whole cell lysate)
A: HepG2
7.5% SDS PAGE
GTX107343 diluted at 1:5000
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