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KSHV ORF73 antibody [AT4C11]

Anti-KSHV ORF73 antibody [AT4C11] used in Western Blot (WB). GTX50023
Anti-KSHV ORF73 antibody [AT4C11] used in IHC (Paraffin sections) (IHC-P). GTX50023

Cat No. GTX50023

Host

Mouse

Clonality

Monoclonal

Clone Name

AT4C11

Isotype

IgG2a

Application

WB, IHC-P, ELISA

Reactivity

Kaposi's sarcoma-associated herpesvirus
Package
100 μl ($319)

APPLICATION

Application Note

We recommend the following starting dilutions:
Western blot is 1:1,000 ~ 1:2,000
Immunohistochemistry: Use at 1:50~100.

Optimal working concentrations should be determined experimentally by the end user.

PROPERTIES

Form

Liquid

Buffer

Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Kaposi's sarcoma-associated herpesvirus

Immunogen

Recombinant human ORF73/HHV8 (122-329aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Human herpesvirus 8 ORF73 , HHV-8 ORF73 , KSHV ORF73 , Kaposi's sarcoma-associated herpesvirus ORF73

Background

ORF73 protein of human herpesvirus 8(HHV-8) is a kaposi's sarcoma-associated herpesvirus (kSHV) latent protein which has been suspected of playing a crucial role in modulating viral and cellular gene expression. ORF73 is associated with cellular chromatin and stays on the chromosomes during cell division. It maintains the viral genomes during cell division by tethering the viral episomes to the chromosomes. It binds directly to replication origin recognition complexes (ORCs) that are primarily associated with the terminal repeat (TR) region of the HHV-8 genome.

Research Area

DATA IMAGES

Anti-KSHV ORF73 antibody [AT4C11] used in Western Blot (WB). GTX50023

GTX50023 WB Image

Transfected and non-transfected 293T cell lysates were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-KSHV ORF73 (1:1,000) and anti-MBP (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HR

Anti-KSHV ORF73 antibody [AT4C11] used in IHC (Paraffin sections) (IHC-P). GTX50023

GTX50023 IHC-P Image

Paraffin embedded sections of Kaposi’s sarcoma tissue were incubated with anti-KSHV ORF73 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf