GeneTex
United States (US)

Kallikrein 11 antibody [N1C3]

GTX104200_WB_19011506_975.jpg
GTX104200_40002_IHC_2_19011506_437.jpg
GTX104200_40002_IHC_19011506_228.jpg
GTX104200_40002_WB_M_brain_19011506_173.jpg

Cat No. GTX104200

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse brain

Predict Reactivity

Rat(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Kallikrein 11. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MGC33060 antibody, PRSS20 antibody, TLSP antibody, KLK11 antibody, kallikrein-11 antibody, hK11 antibody, serine protease 20 antibody, kallikrein 11 antibody, hippostasin antibody, trypsin-like protease antibody, kallikrein-related peptidase 11 antibody

Cellular Localization

Secreted

Background

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Alternate splicing of this gene results in two transcript variants encoding two different isoforms which are differentially expressed. [provided by RefSeq]

Database

DATA IMAGES
GTX104200_WB_19011506_975.jpg

GTX104200 WB Image

Sample (30μg whole cell lysate)
A:MOLT4 (GTX27912)
10% SDS PAGE
GTX104200 diluted at 1:2000

GTX104200_40002_IHC_2_19011506_437.jpg

GTX104200 IHC-P Image

KLK11 antibody [N1C3] detects KLK11 protein at cytosol on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
KLK11 antibody [N1C3] (GTX104200) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104200_40002_IHC_19011506_228.jpg

GTX104200 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using KLK11(GTX104200) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104200_40002_WB_M_brain_19011506_173.jpg

GTX104200 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX104200 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169