GeneTex
United States (US)

Kallikrein 11 (cleaved Ile54) antibody

Kallikrein-11-cleaved-Ile54-antibody-GTX86927-WB-1_18121411_788.jpg

Cat No. GTX86927

Host Rabbit
Clonality Polyclonal
Application WB, ELISA
Reactivity Human
APPLICATION

Application Note

We recommend the following starting dilutions:

WB: 1:500~1:1000
ELISA: 1:10000

Optimal dilutions should be determined experimentally by the end user.

Calculated MW

25 kDa. ( Note )

Specificity/Sensitivity

Kallikrein-11 (Cleaved-Ile54) Antibody detects endogenous levels of fragment of activated Kallikrein-11 resulting from cleavage adjacent to Ile54.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human Kallikrein 11.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Kallikrein Related Peptidase 11,Prss20,Tlsp,Klk11

Background

Possible multifunctional protease. Efficiently cleaves 'bz-Phe-Arg-4-methylcoumaryl-7-amide', a kallikrein substrate, and weakly cleaves other substrates for kallikrein and trypsin. Cleaves synthetic peptides after arginine but not lysine residues.

Database

DATA IMAGES
Kallikrein-11-cleaved-Ile54-antibody-GTX86927-WB-1_18121411_788.jpg

GTX86927 WB Image

Western blot analysis of extracts from HeLa cells, treated with etoposide 25uM 24H, using Kallikrein-11 (Cleaved-Ile54) Antibody. The lane on the right is treated with the synthesized peptide.

Package List Price ($)
$ 339