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Kallikrein 7 antibody

Overexpression
Anti-Kallikrein 7 antibody used in Western Blot (WB). GTX100700
Anti-Kallikrein 7 antibody used in IHC (Paraffin sections) (IHC-P). GTX100700
Anti-Kallikrein 7 antibody used in Western Blot (WB). GTX100700
Anti-Kallikrein 7 antibody used in Western Blot (WB). GTX100700
Anti-Kallikrein 7 antibody used in Western Blot (WB). GTX100700

Cat. No. GTX100700

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Kallikrein 7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , SK-N-SH , IMR32 , Kallikrein7-transfected 293T cells , mouse brain

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.31 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Kallikrein 7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950630

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

kallikrein related peptidase 7 , PRSS6 , SCCE , hK7

Cellular Localization

Secreted

Background

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its encoded enzyme is thought to be involved in the proteolysis of intercellular cohesive structures preceding desquamation, which is the shedding of the outermost layer of the epidermis. Alternative splicing of this gene results in two transcript variants encoding the same protein. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Kallikrein 7 antibody used in Western Blot (WB). GTX100700

GTX100700 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Kallikrein 7 antibody (GTX100700) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Kallikrein 7 antibody used in IHC (Paraffin sections) (IHC-P). GTX100700

GTX100700 IHC-P Image

Kallikrein 7 antibody detects Kallikrein 7 protein at cell membrane and secreted by immunohistochemical analysis.
Sample: Paraffin-embedded mouse skin.
Kallikrein 7 stained by Kallikrein 7 antibody (GTX100700) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Kallikrein 7 antibody used in Western Blot (WB). GTX100700

GTX100700 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Kallikrein 7 antibody (GTX100700) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Kallikrein 7 antibody used in Western Blot (WB). GTX100700

GTX100700 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Kallikrein 7 antibody (GTX100700) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Kallikrein 7 antibody used in Western Blot (WB). GTX100700

GTX100700 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Kallikrein 7 antibody (GTX100700) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169