GeneTex
United States (US)

Kallikrein 7 antibody

GTX103548_43096_20180206_IHC-P_M_19011506_699.jpg
GTX103548_IHC_19011506_789.jpg
GTX103548_43096_20180112_WB_B_19021719_252.jpg

Cat No. GTX103548

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

Kallikrein 7-transfected 293T

Predict Reactivity

Dog, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.69 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Kallikrein 7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Kallikrein Related Peptidase 7,Prss6,Scce,Hk7,Klk7

Cellular Localization

Secreted

Background

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its encoded enzyme is thought to be involved in the proteolysis of intercellular cohesive structures preceding desquamation, which is the shedding of the outermost layer of the epidermis. Alternative splicing of this gene results in two transcript variants encoding the same protein. [provided by RefSeq]

Database

DATA IMAGES
GTX103548_43096_20180206_IHC-P_M_19011506_699.jpg

GTX103548 IHC-P Image

Kallikrein 7 antibody detects secreted Kallikrein 7 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse skin.
Kallikrein 7 stained by Kallikrein 7 antibody (GTX103548) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX103548_IHC_19011506_789.jpg

GTX103548 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using KLK7(GTX103548) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX103548_43096_20180112_WB_B_19021719_252.jpg

GTX103548 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Kallikrein 7 antibody (GTX103548) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169