Ketohexokinase antibody [N1C3]

Cat. No. GTX109591

Reference 10

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human, Mouse, Rat, Pig
Package	100 μl ($399), 25 μl ($169)

See all Ketohexokinase products

View product citations for antibody GTX109591 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:10000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

Mouse liver

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.4 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Ketohexokinase Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950614

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ketohexokinase

Background

This gene encodes ketohexokinase that catalyzes conversion of fructose to fructose-1-phosphate. The product of this gene is the first enzyme with a specialized pathway that catabolizes dietary fructose. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Database

Gene ID: 3795 KHK
UniProt: P50053 KHK

Research Area

DATA IMAGES

GTX109591 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using Ketohexokinase(GTX109591) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX109591 IHC-P Image

Ketohexokinase antibody [N1C3] detects Ketohexokinase protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Ketohexokinase stained by Ketohexokinase antibody [N1C3] (GTX109591) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX109591 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Ketohexokinase antibody [N1C3] (GTX109591) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

GTX109591 IHC-P Image

Ketohexokinase antibody [N1C3] detects Ketohexokinase protein at cytoplasm and nucleus in mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Ketohexokinase antibody [N1C3] (GTX109591) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX109591 WB Image

Sample (20 μg of whole cell lysate)
A: mouse Liver
12% SDS PAGE
GTX109591 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.