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Ki67 antibody [3D11]

Anti-Ki67 antibody [3D11] used in Western Blot (WB). GTX84107
Anti-Ki67 antibody [3D11] used in IHC (Paraffin sections) (IHC-P). GTX84107
Anti-Ki67 antibody [3D11] used in IHC (Paraffin sections) (IHC-P). GTX84107
Anti-Ki67 antibody [3D11] used in IHC (Paraffin sections) (IHC-P). GTX84107
Anti-Ki67 antibody [3D11] used in IHC (Paraffin sections) (IHC-P). GTX84107
Anti-Ki67 antibody [3D11] used in IHC (Paraffin sections) (IHC-P). GTX84107
Anti-Ki67 antibody [3D11] used in IHC (Paraffin sections) (IHC-P). GTX84107
Anti-Ki67 antibody [3D11] used in IHC (Paraffin sections) (IHC-P). GTX84107
Anti-Ki67 antibody [3D11] used in IHC (Paraffin sections) (IHC-P). GTX84107
Anti-Ki67 antibody [3D11] used in IHC (Paraffin sections) (IHC-P). GTX84107
Anti-Ki67 antibody [3D11] used in IHC (Paraffin sections) (IHC-P). GTX84107
Anti-Ki67 antibody [3D11] used in IHC (Paraffin sections) (IHC-P). GTX84107

Cat. No. GTX84107

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3D11

Isotype

 IgG2b

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($519)
See all Ki67 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

359 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein expressed in E.coli corresponding to amino acids 1-250 of human MKI67.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_10728990

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

marker of proliferation Ki-67 , KIA , MIB- , MIB-1 , PPP1R105

Cellular Localization

Nucleus,Nucleolus

Background

Thought to be required for maintaining cell proliferation.

Database

Research Area

DATA IMAGES

Anti-Ki67 antibody [3D11] used in Western Blot (WB). GTX84107

GTX84107 WB Image

WB analysis of HEK293T cells transfected with Ki67 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84107 Ki67 antibody [3D11].
Loading : 5 ug per lane

Anti-Ki67 antibody [3D11] used in IHC (Paraffin sections) (IHC-P). GTX84107

GTX84107 IHC-P Image

IHC-P analysis of pancreas tissue using GTX84107 Ki67 antibody [3D11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-Ki67 antibody [3D11] used in IHC (Paraffin sections) (IHC-P). GTX84107

GTX84107 IHC-P Image

IHC-P analysis of kidney carcinoma tissue using GTX84107 Ki67 antibody [3D11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-Ki67 antibody [3D11] used in IHC (Paraffin sections) (IHC-P). GTX84107

GTX84107 IHC-P Image

IHC-P analysis of pancreas carcinoma tissue using GTX84107 Ki67 antibody [3D11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-Ki67 antibody [3D11] used in IHC (Paraffin sections) (IHC-P). GTX84107

GTX84107 IHC-P Image

IHC-P analysis of colon adenocarcinoma tissue using GTX84107 Ki67 antibody [3D11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-Ki67 antibody [3D11] used in IHC (Paraffin sections) (IHC-P). GTX84107

GTX84107 IHC-P Image

IHC-P analysis of lung carcinoma tissue using GTX84107 Ki67 antibody [3D11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-Ki67 antibody [3D11] used in IHC (Paraffin sections) (IHC-P). GTX84107

GTX84107 IHC-P Image

IHC-P analysis of breast adenocarcinoma tissue using GTX84107 Ki67 antibody [3D11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-Ki67 antibody [3D11] used in IHC (Paraffin sections) (IHC-P). GTX84107

GTX84107 IHC-P Image

IHC-P analysis of ovary tissue using GTX84107 Ki67 antibody [3D11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-Ki67 antibody [3D11] used in IHC (Paraffin sections) (IHC-P). GTX84107

GTX84107 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX84107 Ki67 antibody [3D11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-Ki67 antibody [3D11] used in IHC (Paraffin sections) (IHC-P). GTX84107

GTX84107 IHC-P Image

IHC-P analysis of colon tissue using GTX84107 Ki67 antibody [3D11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-Ki67 antibody [3D11] used in IHC (Paraffin sections) (IHC-P). GTX84107

GTX84107 IHC-P Image

IHC-P analysis of thyroid carcinoma tissue using GTX84107 Ki67 antibody [3D11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-Ki67 antibody [3D11] used in IHC (Paraffin sections) (IHC-P). GTX84107

GTX84107 IHC-P Image

IHC-P analysis of endometrium tissue using GTX84107 Ki67 antibody [3D11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

REFERENCE

Application Reference

Xu D et al. Eur Rev Med Pharmacol Sci 2018; 22 (21) : 7431-7438 Effects of miR-155 on hypertensive rats via regulating vascular mesangial hyperplasia.
Application : WB
Reactivity : Rat
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519