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Ki67 antibody [8D5]

Anti-Ki67 antibody [8D5] used in IHC (Paraffin sections) (IHC-P). GTX82777
Anti-Ki67 antibody [8D5] used in Western Blot (WB). GTX82777

Cat. No. GTX82777

Reference 6

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 8D5

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr, ELISA

Reactivity

 Human, Mouse, Rat, Rabbit
Package
100 μl ($399)
See all Ki67 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF Assay dependent
IHC-P 1/200 - 1/1000
IHC-Fr Assay dependent
ELISA 1/10000
Not tested in other applications.

Calculated MW

359 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to aa (CEDLAGFKELFQTPG) of human KI67, conjugated to KLH.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

marker of proliferation Ki-67 , KIA , MIB- , MIB-1 , PPP1R105

Cellular Localization

Nucleus,Nucleolus

Background

This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009]

Database

Research Area

DATA IMAGES

Anti-Ki67 antibody [8D5] used in IHC (Paraffin sections) (IHC-P). GTX82777

GTX82777 IHC-P Image

IHC-P analysis of lung cancer (left) and rectal cancer (right) using GTX82777 Ki67 antibody [8D5].

Anti-Ki67 antibody [8D5] used in Western Blot (WB). GTX82777

GTX82777 WB Image

WB analysis of HeLa (1), MCF-7 (2) and Raji (3) cell lysate using GTX82777 Ki67 antibody [8D5].

REFERENCE

Application Reference

Hsia Y et al. Int J Nanomedicine 2023; 18 : 4253-4274 A Dual Concentration-Tailored Cytokine-Chemo Nanosystem to Alleviate Multidrug Resistance and Redirect Balance of Cancer Proliferation and Apoptosis.

Clone Reference

BX Ho et al. Stem Cell Res Ther 2022; 13 (1):529 Robust generation of human-chambered cardiac organoids from pluripotent stem cells for improved modelling of cardiovascular diseases
Application : IHC-Fr
K Dulka et al. IBRO Neurosci Rep 2021; 10:119-129 Quantitative morphometric and cell-type-specific population analysis of microglia-enriched cultures subcloned to high purity from newborn rat brains
Application : ICC/IF
Reactivity : Rat
F Mascadri et al. J Histochem Cytochem 2021; 69 (8):535-541 Background-free Detection of Mouse Antibodies on Mouse Tissue by Anti-isotype Secondary Antibodies
Application : IHC-P
Reactivity : Mouse
Y Motooka et al. Semin Thorac Cardiovasc Surg 2020; 32 (3):570-578 Endobronchial ultrasound-guided radiofrequency ablation of lung tumors and mediastinal lymph nodes: a preclinical study in animal lung tumor and mediastinal adenopathy models
Application : IHC-P
Reactivity : Rabbit
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399